The study was conducted on crossbred cows under the ICAR-ETT
project, Post-graduate Institute, Department of Animal Reproduction
and Gynaecology Dr. PDKV, Akola.Presynchronization of oestrus was
done in cows by PGF2 alpha (Lutalyse) 25 mg administered im.
Artificial insemination with frozen semen of progeny tested bull
(H.F. x Gir 50 per cent) was carried on at an interval of 12 h.
Flushing of the donor cows for embryo recovery was carried out on
day 7 after last insemination.The time required for onset of
superovulatory oestrus from last PGF2 alpha ranged from 38 to 44 h.
with an average of 38.66 SE 1.73 h.The length of superovulatory
oestrus ranged from 16 to 36 h. with an average of 30 SE 4.76 h.The
average number of corpora lutea and unovulatory follicles in the
donor cows was 12 SE 5.5 and 2.75 SE 0.85 respectively. The average
number of corpora lutea and unovulatory follicles on right and left
ovary recorded was 6.5 SE 2.95, 5.5 SE 2.62 and 1.75 SE 0.47; 1.0
SE 0.40, respectively. Total number of embryos recovered from the
donor cows was 13 with an average of 3.25 SE 2.01 for each donor
cow, out of which 6 embryos were with arrested growth and 7 were
degenerated ones.
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