The development of recombinant DNA technology has made a marked
impact on molecular virology. The cleavage of viral DNA genomes
with restriction enzymes and the cloning of such DNA fragments in
bacterial p1asmids has led to the amplification of selected viral
DNA fragments for sequencing and gene expression. RNA virus genomes
which can be transcribed to their cDNA form were also cloned in
bacterial p1asmids, facilitating the study of RNA virus genes. With
the elucidation in recent years of the promoter sequence of various
viral genes and the expression of these genes in bacteria or yeast,
the understanding of many viral gene functions has made great
progress. Cloning and expression of viral genes in mammalian cells
was made possible by the construction of shuttle plasmid vectors
which carry the origins of DNA replication from bacteria and/or
mammalian viruses. The expression of viral genes in bacteria, yeast
and eukaryotic cells gives reason to hope that it will be possible
to produce viral antigens in large quantities for use as human or
animal vaccines. The present volume attempts to capture for the
reader some of the high lights of recombinant DNA research in the
field of animal and plant viruses."
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