Cell-free protein expression promises to narrow the technological gap between DNA and protein technologies and provide a platform for broad application of synthetic biology principles in the Life Sciences. It is a rapid and high throughput methodology for the conversion of DNA encoded genetic information into protein-mediated biochemical activities. Cell-Free Protein Synthesis: Methods and Protocols brings together the key opinion leaders of cell-free technology development and provides case studies and detailed protocols for the application of cell-free methodology. Chapters cover the main directions in the development of cell-free technologies including several recently developed cell-free systems, as well as a number of applications of cell-free systems ranging from discovery of biofuel enzymes to in vitro assembly of viruses. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Cell-Free Protein Synthesis: Methods and Protocols seeks to serve a wide variety of scientists with its well-honed methodologies.

Providing new and challenging ways of understanding the medieval in the modern and vice versa, The Medieval Motion Picture: The Politics of Adaptation highlights how medieval aesthetic experience breathes life into contemporary cinema. Engaging with the subject of time and temporality, the essays examine the politics of adaptation and our contemporary entanglement with the medieval: not only in overtly medieval-themed films but also in such diverse genres as thrillers, horror films, performance animation, and even science fiction. Among the films and TV shows discussed are productions such as HBO's award winning series Game of Thrones, Francis Ford Coppola's Bram Stoker's Dracula, Akira Kurosawa's Ran, and M. Night Shyamalan's The Sixth Sense.

Bridging the fields of ecosystem science and landscape ecology, this book integrates Dr. Carol Johnston's research on beaver ecosystem alteration at Voyageurs National Park. The findings about the vegetation, soils, and chemistry of beaver impoundments synthesized in the text provide a cohesive reference useful to wetland scientists, ecosystems and landscape ecologysts, wildlife managers, and students. The beaver, Castor canadensis, is an ecosystem engineer unequaled in its capacity to alter landscapes through browsing and dam building, whose population recovery has re-established environmental conditions that probably existed for millenia prior to its near extirpation by trapping in the 1800s and 1900s. Beavers continue to regain much of their natural range throughout North America, changing stream and forest ecosystems in ways that may be lauded or vilified. Interest in beavers by ecologists remains keen as new evidence emerges about the ecological, hydrological, and biogeochemical effects of beaver browsing and construction. There is a critical need for ecologists and land managers to understand the potential magnitude, persistence, and ecosystem services of beaver landscape transformation. The 88-year record of beaver landscape occupation and alteration documented by Dr. Carol Johnston and colleagues from aerial photography and field work provides a unique resource toward understanding the ecosystem effects and sustainability of beaver activity.

In Leukemia and Lymphoma: Detection of Minimal Residual Disease, hands-on experts describe and discuss the minimal residual disease (MRD) methods they have successfully pioneered for leukemias and lymphomas. They apply reverse transcription PCR (RT-PCR) to acute myeloid leukemia (AML), chronic myelogenous leukemia (CML), and acute promyelocytic leukemia (APL). Other PCR methods are used for Non-Hodgkin's Lymphoma and for the monitoring of follicular lymphoma. Additional chapters address the use of real-time quantitative PCR (RQ-PCR), the emergent method of choice, in patients with acute lymphoblastic leukemia (ALL), the evaluation of MRD techniques in clinical trials, and the application of flow cytometry techniques.

Johnston argues that the preemptive first-use of nuclear weapons, long the foundation of American nuclear strategy, was not the carefully reasoned response to a growing Soviet conventional threat. Instead, it was part of a process of cultural 'socialization', by which the United States reconstituted the previously nationalist strategic cultures of the European allies into a seamless western community directed by Washington. Building a bridge between theory and practice, this book examines the usefulness of cultural theory in international history.

Analytical techniques are powerful tools in a chemist's armoury. Spectroscopic data and chemical information are used routinely in laboratories to follow a chemical reaction or elucidate a chemical structure. However, the sophistication of the analytical techniques used changes rapidly, hence the routinely used method of today can all too readily be superseded by the new technology of tomorrow. More Modern Chemical Techniques identifies some applications of the important chemical techniques in use today that are less well known in schools and colleges and which illustrate how chemistry is using state-of-the-art technology to push back the frontiers of the subject. Examples include: elemental analysis such as atomic absorption spectrometry and inductively coupled plasma techniques; separations including electrophoresis, structure determination (eg x-ray diffraction and optical microscopy); and sampling and sample preparation.

Originally published in 1976, this book is a sociological and historical study of class and race relations in a crucial sector of South Africa - the gold mining industry, during and following the First World War. The author develops a Marxist structuralist explanation of the system of racial discrimination, and then goes in to examine the significant historical events of this formative period, notably those surrounding the strike and uprising of the white workers in 1922. The book explains a system of racial domination essentially in terms of the class positions and problems of the dominating groups, and examines historical developments concerning race in terms of class.

Overviews the background, plot, themes, and language of Beowulf Perhaps the most important work written in Old English, Beowulf tells of a world very different from our own. While the history and culture behind the poem make it challenging for modern audiences, its story of war, violence, and heroism remains relevant to today's readers. Though largely neglected until the 20th century, Beowulf is now widely studied by school students and undergraduates. In addition, it continues to shape contemporary popular culture. This companion overviews the poem and its legacy. The first part of the book provides information of interest to a wide range of readers, while the second covers more specialized topics. Thus the initial chapters review the merits of different translations and offer a detailed plot summary, while later chapters discuss the poem's language and style, its treatment of religion, its relation to Anglo-Saxon culture, and its legacy in popular culture. One of the greatest Beowulf scholars was J.R.R. Tolkien, and the book gives special attention to his use of the poem in his own fiction. valuable guide to one of the most challenging yet enduring works of English literature.; Accessible to school students and general readers; Provides a detailed plot summary; Discusses the poem's background, style, and language; Discusses the poem's lasting influence on contemporary popular culture, including its influence on Tolkien

Exploring Cognition: Damaged Brains and Neural Networks analyses the contribution made by cognitive neuropsychology and connectionist modelling to theoretical explanations of cognitive processes. Bringing together evidence from both damaged brains and neural networks, this exciting and innovative approach leads to re-evaluation of traditional theories: connectionist models lesioned to mimic the residual function of the damaged brain and rehabilitated to simulate the process of recovery suggest underlying mechanisms and challenge previous interpretations.
In this reader key articles by leading international researchers are combined with linking commentaries that provide a context, highlight the conceptual themes and evaluate the evidence. Carefully selected to include hotly debated topics, the papers cover, among others, the controversies surrounding explanations for category specificity in object recognition and for covert recognition of faces and words; the mechanisms underlying the use of regular and irregular past tenses; and the reading of regularly and irregularly spelled words. The challenges posed by connectionist models to assumptions about the nature of dissociations, the need for symbolic rule-based operations in language processing and the modularity and localisation of processes are assessed.
Exploring Cognition: Damaged Brains and Neural Networks will be of interest to advanced undergraduates, postgraduates and researchers in cognitive neuropsychology and cognitive neuroscience.

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Excavating English is a curriculum designed for ages 12 and up. It explores the multicultural roots of the English language from 4000 BC to present day. The text provides over 150 pages of information, trivia, word games, activities and Internet links (including a playlist of supplemental videos). The first chapters give an introduction to linguistics, phonetics and phonology. The text then traces English from the shores of the Black Sea (Proto-Indo-European) through the history of the Germanic peoples, the Danes, the Anglo-Saxons, the Normans, and the great wordsmiths of the Renaissance. The history continues as English crosses the ocean to America, then is influenced by immigrants from all over the world. The text is not only informative, but is lively and interesting, and the activities included after each chapter make the curriculum very interactive.

Cell-free protein expression promises to narrow the technological gap between DNA and protein technologies and provide a platform for broad application of synthetic biology principles in the Life Sciences. It is a rapid and high throughput methodology for the conversion of DNA encoded genetic information into protein-mediated biochemical activities. Cell-Free Protein Synthesis: Methods and Protocols brings together the key opinion leaders of cell-free technology development and provides case studies and detailed protocols for the application of cell-free methodology. Chapters cover the main directions in the development of cell-free technologies including several recently developed cell-free systems, as well as a number of applications of cell-free systems ranging from discovery of biofuel enzymes to in vitro assembly of viruses. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Cell-Free Protein Synthesis: Methods and Protocols seeks to serve a wide variety of scientists with its well-honed methodologies.

Providing new and challenging ways of understanding the medieval in the modern and vice versa, this volume highlights how medieval aesthetic experience breathes life into contemporary cinema. Engaging with the subject of time and temporality, the essays examine the politics of adaptation and our contemporary entanglement with the medieval.

Environmental temperature varies in time and space on timescales ranging from a few hours to long-term climate change. Organisms are therefore continually challenged to regulate and maintain functional capacities as their thermal environment changes. This volume brings together many of the leading workers in thermal biology, with backgrounds spanning the disciplines of molecular biology, cell biology, physiology, zoology, ecology and evolutionary biology, to discuss the responses of a wide range of species to temperature change at all scales of organization, ranging through the molecular, cellular, organismal, population and ecosystem levels. The volume provides an important and comprehensive contribution to the study of temperature adaptation, which, given the concern about global climate change, will provide much to interest a wide range of biologists.

Environmental temperature varies in time and space on timescales ranging from a few hours to long-term climate change. Organisms are therefore continually challenged to regulate and maintain functional capacities as their thermal environment changes. This volume brings together many of the leading workers in thermal biology, with backgrounds spanning the disciplines of molecular biology, cell biology, physiology, zoology, ecology and evolutionary biology, to discuss the responses of a wide range of species to temperature change at all scales of organization, ranging through the molecular, cellular, organismal, population and ecosystem levels. The volume provides an important and comprehensive contribution to the study of temperature adaptation, which, given the concern about global climate change, will provide much to interest a wide range of biologists.

Today's North Atlantic Treaty Organization, with nearly thirty members and a global reach, differs strikingly from the alliance of twelve created in 1949 to "keep the Americans in, the Russians out, and the Germans down." These differences are not simply the result of the Cold War's end, 9/11, or recent twenty-first-century developments but represent a more general pattern of adaptability first seen in the incorporation of Germany as a full member of the alliance in the early 1950s. Unlike other enduring post-World War II institutions that continue to reflect the international politics of their founding era, NATO stands out for the boldness and frequency of its transformations over the past seventy years. In this compelling book, Seth A. Johnston presents readers with a detailed examination of how NATO adapts. Nearly every aspect of NATO-including its missions, functional scope, size, and membership-is profoundly different than at the organization's founding. Using a theoretical framework of "critical junctures" to explain changes in NATO's organization and strategy throughout its history, Johnston argues that the alliance's own bureaucratic actors played important and often overlooked roles in these adaptations. Touching on renewed confrontation between Russia and the West, which has reignited the debate about NATO's relevance, as well as a quarter century of post-Cold War rapprochement and more than a decade of expeditionary effort in Afghanistan, How NATO Adapts explores how crises from Ukraine to Syria have again made NATO's capacity for adaptation a defining aspect of European and international security. Students, scholars, and policy practitioners will find this a useful resource for understanding NATO, transatlantic relations, and security in Europe and North America, as well as theories about change in international institutions.

Phosphorus is an essential element for plant growth and its input has long been recognized as necessary to maintain profitable crop production. However, phosphorus inputs can also increase the biological activity of surface waters and this can lead to the destruction of such aquatic ecosystems. Advanced eutrophication of surface water leads to problems with its use for fisheries, recreation, industry and drinking, due to the increased growth of undesirable algae and aquatic weeds, and oxygen shortages caused by their death and decomposition. It is therefore important to have a good understanding of the causes and mode of phosphorus loss from soil to water, to enable the problems to be controlled and managed. This book has been developed from an international workshop held in Ireland in late 1995. It is the first comprehensive consideration of the topic, and many leading researchers in the area have contributed to it. It is essential reading for all soil scientists and freshwater biologists, as well as for environmentalists, ecologists and agriculturalists concerned with sustainable land management and pollution. The book is also recommended to fisheries managers, fertilizer manufacturers and biologists working for water utilities.

In Leukemia and Lymphoma: Detection of Minimal Residual Disease, hands-on experts describe and discuss the minimal residual disease (MRD) methods they have successfully pioneered for leukemias and lymphomas. They apply reverse transcription PCR (RT-PCR) to acute myeloid leukemia (AML), chronic myelogenous leukemia (CML), and acute promyelocytic leukemia (APL). Other PCR methods are used for Non-Hodgkin's Lymphoma and for the monitoring of follicular lymphoma. Additional chapters address the use of real-time quantitative PCR (RQ-PCR), the emergent method of choice, in patients with acute lymphoblastic leukemia (ALL), the evaluation of MRD techniques in clinical trials, and the application of flow cytometry techniques.

Johnston argues that the preemptive first-use of nuclear weapons, long the foundation of American nuclear strategy, was not the carefully reasoned response to a growing Soviet conventional threat. Instead, it was part of a process of cultural 'socialization', by which the United States reconstituted the previously nationalist strategic cultures of the European allies into a seamless western community directed by Washington. Building a bridge between theory and practice, this book examines the usefulness of cultural theory in international history.

Alexander and James Campbell emigrated from Scotland to the United States as teenagers in the 1850s and settled in vastly different regions of the country-Alexander in New York City and James in Charleston, South Carolina. When the Civil War broke out, Alexander and James opted to fight for their adopted states and causes: Alexander enlisted in the 79th New York "Highlanders" and James in the 1st South Carolina ("Charleston") Battalion. "Him on the One Side and Me on the Other" tells the remarkable story of these two brothers through nearly eighty wartime letters to family and to each other. In this moving chronicle, Terry A. Johnston, Jr., follows the brothers as they endure the hardships of war and react to their plight. Alexander accompanied his regiment on campaigns through Virginia, South Carolina, and Kentucky and was wounded in battle while bearing the regimental colors. James served with distinction near Charleston, was captured at the Battle of Battery Wagner in July 1863, and spent the final two years of the war in three Northern prisons. In the most dramatic moment in this story of the brothers' wartime experiences, the letters reveal a near-reunion on the battlefield of Secessionville, South Carolina, on June 16, 1862, where Alexander was part of the Union force that assaulted Tower Battery, a fort inhabited by James and his Confederate comrades.