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Showing 1 - 3 of 3 matches in All Departments
Whiteflies, taxonomy biotypes. Management on whitefly. Whiteflies - vector of viruses. Whitefly - Plant Relationship. Responsible for crop failures throughout the globe. Whitefly in Agricultural System. Developed resistance to pesticides with the resurgence.
A high sucrose clone S. officinarum 'Gungera' and a low sucrose clone S. spontaneum 'SES 603' were crossed to obtain interspecific hybrids named as ISH-1 to ISH-29 and graded into very high, high, low and very low sucrose types The objectives of present investigation were to compare high and low sugar genotypes, as also, their progeny for sucrose content, and to identify molecular markers for sucrose accumulation in sugarcane. Simultaneous work was carried on six selected commercial sugarcane cultivars also. Protein markers obtained in SDS-PAGE showed presence of a protein band of MW 19.95 kD in high sucrose types. Commercial sugarcane var. Co 7717 showed presence of a total 37 bands, of which protein bands of MW 100, 87.09, 83.17 and 81.28 kD were highly specific. DNA markers for high and low sucrose were identified. Of the ten microsatellite primers selected, four SSRs distinguished high and low sucrose markers. SSR primer AI, A and B amplified high sucrose markers and SSR primer SMC226CG+ SMC226CG amplified a low sucrose marker.
Differentiation i.e. development of single cell into complex multicellular organs & tissues, results through selective gene expression which is specific to cell type, region & organ. Therefore, identification of biochemical markers during sequential stages of organogenesis could be used to identify early events of differentiation, embryogenic potential or serve as an indication of loss of regeneration capacity with culture age. Keeping in view these facts, this M. Phil. dissertation on T. indica, an important medicinal plant comprises of six chapters: Chapter-1 highlights background for selection of this plant species; Chapter-2 is compilation of relevant updated literature in the area of tissue culture, biochemical & SDS-PAGE studies; Chapter-3 deals with details of materials & techniques used; Chapter-4 presents the findings of study supported by tables, photographs & illustrations; Chapter-5 discusses the results in the light of available literature and Chapter-6 is summary of work followed by literature cited. This M. Phil. dissertation will be useful for reseachers working in the area of plant tissue culture & cell differentiation.
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