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Sucrose synthesis, transport and accumulation in Saccharum genotypes (Paperback)
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Sucrose synthesis, transport and accumulation in Saccharum genotypes (Paperback)
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A high sucrose clone S. officinarum 'Gungera' and a low sucrose
clone S. spontaneum 'SES 603' were crossed to obtain interspecific
hybrids named as ISH-1 to ISH-29 and graded into very high, high,
low and very low sucrose types The objectives of present
investigation were to compare high and low sugar genotypes, as
also, their progeny for sucrose content, and to identify molecular
markers for sucrose accumulation in sugarcane. Simultaneous work
was carried on six selected commercial sugarcane cultivars also.
Protein markers obtained in SDS-PAGE showed presence of a protein
band of MW 19.95 kD in high sucrose types. Commercial sugarcane
var. Co 7717 showed presence of a total 37 bands, of which protein
bands of MW 100, 87.09, 83.17 and 81.28 kD were highly specific.
DNA markers for high and low sucrose were identified. Of the ten
microsatellite primers selected, four SSRs distinguished high and
low sucrose markers. SSR primer AI, A and B amplified high sucrose
markers and SSR primer SMC226CG+ SMC226CG amplified a low sucrose
marker.
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