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The book presents the latest advances and research findings in the
fields of computational science and communication. The areas
covered include smart innovation; systems and technologies;
embedded knowledge and intelligence; innovation and sustainability;
advanced computing; and networking and informatics. It also focuses
on the knowledge-transfer methodologies and the innovation
strategies employed to make these effective. This fascinating
compilation appeals to researchers, academics and engineers around
the globe.
This book presents the latest advances and research findings in the
fields of computational science and communication presented at the
International Conference on Smart Innovations in Communications and
Computational Sciences (ICSICCS 2020). The areas covered include
smart innovation; systems and technologies; embedded knowledge and
intelligence; innovation and sustainability; advanced computing;
networking and informatics. It also focuses on the
knowledge-transfer methodologies and the innovation strategies
employed to make these effective. This fascinating compilation
appeals to researchers, academics and engineers around the globe.
The objective of this study was to understand the pitfalls of the
poor oocyte cleavage and subsequent embryo production by assessing
the developmental competence of the oocytes in buffalo using
parthenogenesis as a model which was compared with IVF (natural
activation). Buffalo ovaries were collected from slaughter house
and matured oocytes were subjected to either IVF (control) or
chemical activation (treatment) 7% ethanol (ET)for 7 min ] 2.5 mM
6-Di methyl amino purine (6-DMAP) for 4 hr, or 7% ET for 7 min + 10
ug/ml Cycloheximide (CHX) for 6 hr or 7% ET for 7 min + 2.5 mM
6-DMAP + 10 ug/ml CHX for 6 hr]. Fertilized and chemically
activated oocytes were cultured in mSOF medium for 8 days to study
embryo development.In conclusion, the results of parthenogenesis,
revealed that buffalo oocytes had better inherent developmental
competence and the poor cleavage and embryo development following
IVF, in our study, may partly be due to the poor quality of
frozen-thawed sperm, improper sperm capacitation and/ or
fertilization. Further, high ambient temperature adversely affected
the cleavage and embryo development following IVF.
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