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Different bacteria were isolated during the study including E.
coli, Pseudomonas spp., Citrobacter spp., Enterobacter spp.,
Morganella spp., Proteus spp., Providencia, Staph. aureus, and
Staph. epidermidis. Then check the susceptibility of these bacteria
against different antibiotic like Augmentin (a combination of
Amoxicillin and Clavulanic Acid), Amoxil (Amoxicillin), Velocef
(Cephradine), Cefizix (Ceftizoxime Sodium), Ciproxin
(Ciprofloxacin), Erythrocin (Erythromycin), Meronem (Meropenem),
Gentacin (Gentamycin), Vibramycin (Doxycycline), Vancocin
(Vancomycin), Septran (a combination of Trimethoprim and
Sulphamethoxazole), Sulzone (a combination of Cefoprazone and
Sulbactam)and Tazocin (a combination of Piperacillin and
Tazobactam). The studies showed that Meronem is the most effective
drug against E. coli having 65.78% activity however E. coli showed
97.36% resistance against Erythrocin. The most effective drug
against Pseudomonas spp. was Gentacin which was 83.33% sensitive
and Pseudomonas spp. showed 91.66% resistance against Augmentin,
Erythrocin and Vancocin. Citrobacter spp. showed 100% resistance
against Vancocin. The most effective drug among there was
Vibramycin 63.63%.
In the current study different samples were collected from computer
accessories used in the Hazara University and different diagnostic
laboratories of Mansehra city. 150 samples were examined for their
total bacterial count and the maximum growths were observed from
the samples collected. Similarly the samples were analyzed further
for the identification of different micro-organisms such as E.coli,
Klebsiella, S.aureus, S.epidermidis and these organisms were found
to be present. The percentage of micro-organisms were 46.66% of
E.coli, 20% of Klebsiella, 16.66 of S.aureus and, 16.66% of
S.epidermidis were detected and identified on the selective media
i.e. E.M.B. agar and Mannitol salt agar respectively. Furthermore,
biochemical tests were performed to confirm their presence i.e.
IMVIC Test, Catalase Test, Coagulase Tests were also performed for
their identification. The identified micro-organisms were again
checked for their susceptibility against different standard
antibiotics and their zone of inhibition were measured and noticed.
Some of the E.coli, Klebsiella and S.aureus showed reasonable
resistance against the antibiotics used.
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