In ELISA formats there are several parameters that are common and critical to assay performance and should be considered during the optimization process, including antibodies concentration, antigen concentration, incubation time of blocking, substrate solution and washing solution. Though immunoassays offer a specific, sensitive and rapid method to detect and quantify even trace amounts of allergens in foodstuffs, certain processes can denature, alter or destroy proteins so that they are no longer detectible by assay using antibodies. When using monoclonal antibodies, there is a great chance of obtaining false negatives in the detection. Polyclonal antibodies are recommended for ELISA techniques as the risk of obtaining false negatives is reduced as compared to monoclonal antibodies which are quite specific.

Chrysoperla carnea, known as the common green lacewing, is an insect in the Chrysopidae family. It is found in many parts of North America, Europe and Asia. The adults feed on nectar, pollen and aphid honeydew but the larvae are active predators and feed on aphids and other small insects. It has been used in the biological control of insectpests on crops. Chrysoperla carnea was originally considered to be a single species with a holarctic distribution but it has now been shown to be a complex of many cryptic, sibling species. These are indistinguishable from each other morphologicallybut can be recognised by variations in the vibrational songs the insects use to communicate with each other, which they especially do during courtship. The green lacewing eggs are oval and secured to the plant by long slender stalks. They are pale green when first laid but become gray later. The larvae are about one millimetre long when they first hatch. They are brown and resemble small alligators, crawling actively around in search of prey. They have a pair of pincer-like mandibles on their head with which they grasp their prey, sometimes lifting the victim off the leaf surface to prevent its escape.

While studies specifying generic integration architectures are abundant, little or no studies are dedicated for applications performance over such heterogeneous networks. The purpose of this research is to evaluate the performance of integrated UMTS and WLAN network by using two types of standard integrated models proposed by 3GPP, open coupling and loose coupling together with network layer mobility management scheme such as Mobile IP.In this research we calculate the different application's traffic our concluded results shows that page response time is smaller for loose coupling scheme as compare to open coupling scheme although difference is not so much wide, pageresponse time increases with the passage of time because number of users increases as the time passes. Same is In case for FTP, download response time for FTP is smaller in case of loose coupling scheme. We have also calculated the WLAN performance parameters (delay and throughput) and UMTS downlink traffic sent and received. We have discussed in detail architecture and functionalities of UMTS and WLAN, mobile IP, standard interworked UMTS and WLAN models proposed by 3GPP.