|
Showing 1 - 3 of
3 matches in All Departments
With the completion of sequencing projects and the advancement of
a- lytical tools for protein identification, proteomics-the study
of the expressed part of the genome-has become a major region of
the burgeoning field of functional genomics. High-resolution 2-D
gels can reveal virtually all p- teins present in a cell or tissue
at any given time, including posttranslationally modified proteins.
Changes in the expression and structure of most cellular proteins
caused by differentiation or external stimuli can be displayed and
eventually identified using 2-D protein gels. 2-D Proteome Analysis
Protocols covers all aspects of the use of 2-D protein
electrophoresis for the analysis of biological problems. The
contri- tors include many of the leaders in the fields of
biochemistry and analytical chemistry who were instrumental in the
development of high-resolution 2-D gels, immobilized pH gradients,
computer analysis, and mass spectromet- based protein
identification methodologies. This book is intended as a benchtop
manual and guide both for novices to 2-D gels and for those
aficionados who wish to try the newer techniques. Any group using
protein biochemistry-especially in the fields of molecular biology,
biochemistry, microbiology, and cell biology-should find this book
eminently useful. 2-D Proteome Analysis Protocols takes the
researcher through the c- plete process of working with 2-D protein
gels from making the protein - tract to finally identifying the
proteins of interest. It includes protocols for generating 2-D
protein extracts from most of the standard model organisms,
including bacteria, yeast, nematode, Drosophila, plants, mouse, and
human.
With the completion of sequencing projects and the advancement of
a- lytical tools for protein identification, proteomics-the study
of the expressed part of the genome-has become a major region of
the burgeoning field of functional genomics. High-resolution 2-D
gels can reveal virtually all p- teins present in a cell or tissue
at any given time, including posttranslationally modified proteins.
Changes in the expression and structure of most cellular proteins
caused by differentiation or external stimuli can be displayed and
eventually identified using 2-D protein gels. 2-D Proteome Analysis
Protocols covers all aspects of the use of 2-D protein
electrophoresis for the analysis of biological problems. The
contri- tors include many of the leaders in the fields of
biochemistry and analytical chemistry who were instrumental in the
development of high-resolution 2-D gels, immobilized pH gradients,
computer analysis, and mass spectromet- based protein
identification methodologies. This book is intended as a benchtop
manual and guide both for novices to 2-D gels and for those
aficionados who wish to try the newer techniques. Any group using
protein biochemistry-especially in the fields of molecular biology,
biochemistry, microbiology, and cell biology-should find this book
eminently useful. 2-D Proteome Analysis Protocols takes the
researcher through the c- plete process of working with 2-D protein
gels from making the protein - tract to finally identifying the
proteins of interest. It includes protocols for generating 2-D
protein extracts from most of the standard model organisms,
including bacteria, yeast, nematode, Drosophila, plants, mouse, and
human.
Based on a popular course at Cold Spring Harbor Laboratory, this
new manual assembles cutting-edge protocols, helpful hints, and
lecture notes to teach researchers from a wide variety of
disciplines the essential methods of proteomics using
state-of-the-art instrumentation. Detailed protocols involving
protein microarrays, liquid chromatography, high-throughput cloning
of expression constructs, IMAC, mass spectrometry, MALDI-TOF, and
MudPIT are provided, along with well-illustrated descriptions of
experimental procedures and lists of recommended websites and
reading material. Proteomics: A Cold Spring Harbor Laboratory
Course Manual can be used both as the basis for a course and as a
detailed bench manual for those performing indispensable proteomic
experiments. It is authored by Andrew J. Link, Philip C. Andrews,
and Joshua LaBaer, all leaders in their fields, who bring
complementary expertise to the manual.
|
You may like...
Loot
Nadine Gordimer
Paperback
(2)
R398
R330
Discovery Miles 3 300
|