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2-D Proteome Analysis Protocols (Hardcover, 1999 ed.)
Loot Price: R4,411
Discovery Miles 44 110
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2-D Proteome Analysis Protocols (Hardcover, 1999 ed.)
Series: Methods in Molecular Biology, 112
Expected to ship within 12 - 17 working days
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With the completion of sequencing projects and the advancement of
a- lytical tools for protein identification, proteomics-the study
of the expressed part of the genome-has become a major region of
the burgeoning field of functional genomics. High-resolution 2-D
gels can reveal virtually all p- teins present in a cell or tissue
at any given time, including posttranslationally modified proteins.
Changes in the expression and structure of most cellular proteins
caused by differentiation or external stimuli can be displayed and
eventually identified using 2-D protein gels. 2-D Proteome Analysis
Protocols covers all aspects of the use of 2-D protein
electrophoresis for the analysis of biological problems. The
contri- tors include many of the leaders in the fields of
biochemistry and analytical chemistry who were instrumental in the
development of high-resolution 2-D gels, immobilized pH gradients,
computer analysis, and mass spectromet- based protein
identification methodologies. This book is intended as a benchtop
manual and guide both for novices to 2-D gels and for those
aficionados who wish to try the newer techniques. Any group using
protein biochemistry-especially in the fields of molecular biology,
biochemistry, microbiology, and cell biology-should find this book
eminently useful. 2-D Proteome Analysis Protocols takes the
researcher through the c- plete process of working with 2-D protein
gels from making the protein - tract to finally identifying the
proteins of interest. It includes protocols for generating 2-D
protein extracts from most of the standard model organisms,
including bacteria, yeast, nematode, Drosophila, plants, mouse, and
human.
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