A comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids, recombinant protein expression, and the use of reporter genes, are also described.

A wide-ranging anthology of primary texts in American foreign relations—now expanded to include documents from the Trump years to today How should America wield its power beyond its borders? Should it follow grand principles or act on narrow self-interest? Should it work in concert with other nations or avoid entangling alliances? America in the World captures the voices and viewpoints of some of the most provocative, eloquent, and influential people who participated in these and other momentous debates. Now fully revised and updated, this anthology brings together primary texts spanning a century and a half of U.S. foreign relations, illuminating how Americans have been arguing about the nation’s role in the world since its emergence as a world power in the late nineteenth century. Features more than 250 primary-source documents, reflecting an extraordinary range of views Includes two new chapters on the Trump years and the return of great power rivalries under Biden Sweeps broadly from the Gilded Age to emerging global challenges such as COVID-19 Shares the perspectives of presidents, secretaries of state, and generals as well as those of poets, songwriters, clergy, newspaper columnists, and novelists Also includes non-American perspectives on U.S. power

A wide-ranging rethinking of the many factors that comprise the making of American Grand Strategy. What is grand strategy? What does it aim to achieve? And what differentiates it from normal strategic thought-what, in other words, makes it "grand"? In answering these questions, most scholars have focused on diplomacy and warfare, so much so that "grand strategy" has become almost an equivalent of "military history." The traditional attention paid to military affairs is understandable, but in today's world it leaves out much else that could be considered political, and therefore strategic. It is in fact possible to consider, and even reach, a more capacious understanding of grand strategy, one that still includes the battlefield and the negotiating table while expanding beyond them. Just as contemporary world politics is driven by a wide range of non-military issues, the most thorough considerations of grand strategy must consider the bases of peace and security-including gender, race, the environment, and a wide range of cultural, social, political, and economic issues. Rethinking American Grand Strategy assembles a roster of leading historians to examine America's place in the world. Its innovative chapters re-examine familiar figures, such as John Quincy Adams, George Kennan, and Henry Kissinger, while also revealing the forgotten episodes and hidden voices of American grand strategy. They expand the scope of diplomatic and military history by placing the grand strategies of public health, race, gender, humanitarianism, and the law alongside military and diplomatic affairs to reveal hidden strategists as well as strategies.

A comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids, recombinant protein expression, and the use of reporter genes, are also described.

The Cambridge History of America and the World offers a transformative account of American engagement in the world from 1500 to the present. Representing a new scholarship informed by the transnational turn in the writing of US history and American foreign relations, the four-volume reference work gives sustained attention to key moments in US diplomacy, from the Revolutionary War and the Monroe Doctrine to the US rise as a world power in World War I, World War II and the Cold War. The volumes also cast a more inclusive scholarly net to include transnational histories of Native America, the Atlantic world, slavery, political economy, borderlands, empire, the family, gender and sexuality, race, technology, and the environment. Collectively, they offer essential starting points for readers coming to the field for the first time and serve as a critical vehicle for moving this scholarship forward in innovative new directions.

A wide-ranging rethinking of the many factors that comprise the making of American Grand Strategy. What is grand strategy? What does it aim to achieve? And what differentiates it from normal strategic thought-what, in other words, makes it "grand"? In answering these questions, most scholars have focused on diplomacy and warfare, so much so that "grand strategy" has become almost an equivalent of "military history." The traditional attention paid to military affairs is understandable, but in today's world it leaves out much else that could be considered political, and therefore strategic. It is in fact possible to consider, and even reach, a more capacious understanding of grand strategy, one that still includes the battlefield and the negotiating table while expanding beyond them. Just as contemporary world politics is driven by a wide range of non-military issues, the most thorough considerations of grand strategy must consider the bases of peace and security-including gender, race, the environment, and a wide range of cultural, social, political, and economic issues. Rethinking American Grand Strategy assembles a roster of leading historians to examine America's place in the world. Its innovative chapters re-examine familiar figures, such as John Quincy Adams, George Kennan, and Henry Kissinger, while also revealing the forgotten episodes and hidden voices of American grand strategy. They expand the scope of diplomatic and military history by placing the grand strategies of public health, race, gender, humanitarianism, and the law alongside military and diplomatic affairs to reveal hidden strategists as well as strategies.

Outside In presents the newest scholarship that narrates and explains the history of the United States as part of a networked transnational past. This work tells the stories of Americans who inhabited the border-crossing circuitry of people, ideas, and institutions that have made the modern world a worldly place. Forsaking manifestos of transnational history and surveys of existing scholarship for fresh research, careful attention to concrete situations and transactions, and original interpretation, the vigorous, accomplished historians whose work is collected here show how the transnational history of the United States is actually being written. Ranging from high statecraft to political ferment from below, from the history of religion to the discourse of women's rights, from the political left to the political right, from conservative businessmen to African diaspora radicals, this set of original essays narrates U.S. history in new ways, emphasizing the period from 1870 to the present. The essays in Outside In demonstrate the inadequacy of any unidirectional concept of "the U.S. and the world," although they stress the worldly forces that have shaped Americans. At the same time, these essays disrupt and complicate the very idea of simple inward and outward flows of influence, showing how Americans lived within transnational circuits featuring impacts and influences running in multiple directions. Outside In also transcends the divide between work focusing on the international system of nation-states and transnational history that treats non-state actors exclusively. The essays assembled here show how to write transnational history that takes the nation-state seriously, explaining that governments and non-state actors were never sealed off from one another in the modern world. These essays point the way toward a more concrete and fully internationalized vision of modern American history.

For better or worse, be it militarily, diplomatically, politically, economically, or culturally, Americans have had a profound role in shaping the wider world beyond them. Unsurprisingly, most non-Americans have passionate views about the nature of U.S. foreign policy. America has been a savior to some, a curse to others-and both have good reason to feel that way. And yet, such views are often also based on a caricature of American actions and intentions. For their part, Americans themselves have strong opinions about their role in the world and how it has evolved over time. Yet these views are shrouded as much in myth as they are grounded in fact. American Foreign Relations, then, suffers from being a subject of immense worldwide importance but almost complete misunderstanding; it provokes strong emotions and much debate in newspapers daily, but is accompanied by little comprehension. This Very Short Introduction aims to offer analysis of key events, episodes, crises, and individuals in the making of American foreign relations. It will discuss events such as the Revolutionary War, the Louisiana Purchase, the War of 1812, manifest destiny, the Mexican War, the Civil War, industrialization, the beginnings of globalization, the Spanish-American War, imperialism, the annexation of the Philippines, informal imperialism and the Open Door policy, World War I, isolationism, World War II, the Cold War from its origins to its end (including the Korean and Vietnam Wars), the Iraq Wars, 9/11, and Afghanistan. Such topics will be situated within an analytical narrative that follows chronology generally, but not strictly or comprehensively. ABOUT THE SERIES: The Very Short Introductions series from Oxford University Press contains hundreds of titles in almost every subject area. These pocket-sized books are the perfect way to get ahead in a new subject quickly. Our expert authors combine facts, analysis, perspective, new ideas, and enthusiasm to make interesting and challenging topics highly readable.

Outside In presents the newest scholarship that narrates and explains the history of the United States as part of a networked transnational past. This work tells the stories of Americans who inhabited the border-crossing circuitry of people, ideas, and institutions that have made the modern world a worldly place. Forsaking manifestos of transnational history and surveys of existing scholarship for fresh research, careful attention to concrete situations and transactions, and original interpretation, the vigorous, accomplished historians whose work is collected here show how the transnational history of the United States is actually being written. Ranging from high statecraft to political ferment from below, from the history of religion to the discourse of women's rights, from the political left to the political right, from conservative businessmen to African diaspora radicals, this set of original essays narrates U.S. history in new ways, emphasizing the period from 1870 to the present. The essays in Outside In demonstrate the inadequacy of any unidirectional concept of "the U.S. and the world," although they stress the worldly forces that have shaped Americans. At the same time, these essays disrupt and complicate the very idea of simple inward and outward flows of influence, showing how Americans lived within transnational circuits featuring impacts and influences running in multiple directions. Outside In also transcends the divide between work focusing on the international system of nation-states and transnational history that treats non-state actors exclusively. The essays assembled here show how to write transnational history that takes the nation-state seriously, explaining that governments and non-state actors were never sealed off from one another in the modern world. These essays point the way toward a more concrete and fully internationalized vision of modern American history.

How should America wield its enormous power beyond its borders? Should it adhere to grand principles or act on narrow self-interest? Should it partner with other nations or avoid entangling alliances? Americans have been grappling with questions like these throughout the nation's history, and especially since the emergence of the United States as a major world power in the late nineteenth century. "America in the World" illuminates this history by capturing the diverse voices and viewpoints of some of the most colorful and eloquent people who participated in these momentous debates.

Spanning the era from the Gilded Age to the Obama years, this unique reader collects more than two hundred documents--everything from presidential addresses and diplomatic cables to political cartoons and song lyrics. It encompasses various phases of American diplomatic history that are typically treated separately, such as the First World War, the Cold War, and 9/11. The book presents the perspectives of elite policymakers--presidents, secretaries of state, generals, and diplomats--alongside those of other kinds of Americans, such as newspaper columnists, clergymen, songwriters, poets, and novelists. It also features numerous documents from other countries, illustrating how foreigners viewed America's role in the world.

Ideal for classroom use, "America in the World" sheds light on the complex interplay of political, economic, ideological, and cultural factors underlying the exercise of American power on the global stage.Includes more than two hundred documents from the late nineteenth century to todayLooks at everything from presidential addresses to political cartoons and song lyricsPresents diverse perspectives, from elite policymakers to clergymen and novelistsFeatures documents from outside the United States, illustrating how people in other countries viewed America's role in the world

The third volume of The Cambridge History of America and the World covers the volatile period between 1900 and 1945 when the United States emerged as a world power and American engagements abroad flourished in new and consequential ways. Showcasing the most innovative approaches to both traditional topics and emerging themes, leading scholars chart the complex ways in which Americans projected their growing influence across the globe; how others interpreted and constrained those efforts; how Americans disagreed with each other, often fiercely, about foreign relations; and how race, religion, gender, and other factors shaped their worldviews. During the early twentieth century, accelerating forces of global interdependence presented Americans, like others, with a set of urgent challenges from managing borders, humanitarian crises, economic depression, and modern warfare to confronting the radical, new political movements of communism, fascism, and anticolonial nationalism. This volume will set the standard for new understandings of this pivotal moment in the history of America and the world.