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E. coli Plasmid Vectors - Methods and Applications (Hardcover, 2003 ed.)
Loot Price: R4,971
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E. coli Plasmid Vectors - Methods and Applications (Hardcover, 2003 ed.)
Series: Methods in Molecular Biology, 235
Expected to ship within 12 - 17 working days
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A comprehensive collection of readily reproducible techniques for
the manipulation of recombinant plasmids using the bacterial host
E. coli. The authors describe proven methods for cloning DNA into
plasmid vectors, transforming plasmids into E. coli, and analyzing
recombinant clones. They also include protocols for the
construction and screening of libraries, as well as specific
techniques for specialized cloning vehicles, such as cosmids,
bacterial artificial chromosomes, l vectors, and phagemids. Common
downstream applications such as mutagenesis of plasmids,
recombinant protein expression, and the use of reporter genes, are
also described.
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