A comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids, recombinant protein expression, and the use of reporter genes, are also described.

A wide-ranging rethinking of the many factors that comprise the making of American Grand Strategy. What is grand strategy? What does it aim to achieve? And what differentiates it from normal strategic thought-what, in other words, makes it "grand"? In answering these questions, most scholars have focused on diplomacy and warfare, so much so that "grand strategy" has become almost an equivalent of "military history." The traditional attention paid to military affairs is understandable, but in today's world it leaves out much else that could be considered political, and therefore strategic. It is in fact possible to consider, and even reach, a more capacious understanding of grand strategy, one that still includes the battlefield and the negotiating table while expanding beyond them. Just as contemporary world politics is driven by a wide range of non-military issues, the most thorough considerations of grand strategy must consider the bases of peace and security-including gender, race, the environment, and a wide range of cultural, social, political, and economic issues. Rethinking American Grand Strategy assembles a roster of leading historians to examine America's place in the world. Its innovative chapters re-examine familiar figures, such as John Quincy Adams, George Kennan, and Henry Kissinger, while also revealing the forgotten episodes and hidden voices of American grand strategy. They expand the scope of diplomatic and military history by placing the grand strategies of public health, race, gender, humanitarianism, and the law alongside military and diplomatic affairs to reveal hidden strategists as well as strategies.

A comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids, recombinant protein expression, and the use of reporter genes, are also described.

A wide-ranging anthology of primary texts in American foreign relations—now expanded to include documents from the Trump years to today How should America wield its power beyond its borders? Should it follow grand principles or act on narrow self-interest? Should it work in concert with other nations or avoid entangling alliances? America in the World captures the voices and viewpoints of some of the most provocative, eloquent, and influential people who participated in these and other momentous debates. Now fully revised and updated, this anthology brings together primary texts spanning a century and a half of U.S. foreign relations, illuminating how Americans have been arguing about the nation’s role in the world since its emergence as a world power in the late nineteenth century. Features more than 250 primary-source documents, reflecting an extraordinary range of views Includes two new chapters on the Trump years and the return of great power rivalries under Biden Sweeps broadly from the Gilded Age to emerging global challenges such as COVID-19 Shares the perspectives of presidents, secretaries of state, and generals as well as those of poets, songwriters, clergy, newspaper columnists, and novelists Also includes non-American perspectives on U.S. power

For better or worse, be it militarily, diplomatically, politically, economically, or culturally, Americans have had a profound role in shaping the wider world beyond them. Unsurprisingly, most non-Americans have passionate views about the nature of U.S. foreign policy. America has been a savior to some, a curse to others-and both have good reason to feel that way. And yet, such views are often also based on a caricature of American actions and intentions. For their part, Americans themselves have strong opinions about their role in the world and how it has evolved over time. Yet these views are shrouded as much in myth as they are grounded in fact. American Foreign Relations, then, suffers from being a subject of immense worldwide importance but almost complete misunderstanding; it provokes strong emotions and much debate in newspapers daily, but is accompanied by little comprehension. This Very Short Introduction aims to offer analysis of key events, episodes, crises, and individuals in the making of American foreign relations. It will discuss events such as the Revolutionary War, the Louisiana Purchase, the War of 1812, manifest destiny, the Mexican War, the Civil War, industrialization, the beginnings of globalization, the Spanish-American War, imperialism, the annexation of the Philippines, informal imperialism and the Open Door policy, World War I, isolationism, World War II, the Cold War from its origins to its end (including the Korean and Vietnam Wars), the Iraq Wars, 9/11, and Afghanistan. Such topics will be situated within an analytical narrative that follows chronology generally, but not strictly or comprehensively. ABOUT THE SERIES: The Very Short Introductions series from Oxford University Press contains hundreds of titles in almost every subject area. These pocket-sized books are the perfect way to get ahead in a new subject quickly. Our expert authors combine facts, analysis, perspective, new ideas, and enthusiasm to make interesting and challenging topics highly readable.

How should America wield its enormous power beyond its borders? Should it adhere to grand principles or act on narrow self-interest? Should it partner with other nations or avoid entangling alliances? Americans have been grappling with questions like these throughout the nation's history, and especially since the emergence of the United States as a major world power in the late nineteenth century. "America in the World" illuminates this history by capturing the diverse voices and viewpoints of some of the most colorful and eloquent people who participated in these momentous debates.

Spanning the era from the Gilded Age to the Obama years, this unique reader collects more than two hundred documents--everything from presidential addresses and diplomatic cables to political cartoons and song lyrics. It encompasses various phases of American diplomatic history that are typically treated separately, such as the First World War, the Cold War, and 9/11. The book presents the perspectives of elite policymakers--presidents, secretaries of state, generals, and diplomats--alongside those of other kinds of Americans, such as newspaper columnists, clergymen, songwriters, poets, and novelists. It also features numerous documents from other countries, illustrating how foreigners viewed America's role in the world.

Ideal for classroom use, "America in the World" sheds light on the complex interplay of political, economic, ideological, and cultural factors underlying the exercise of American power on the global stage.Includes more than two hundred documents from the late nineteenth century to todayLooks at everything from presidential addresses to political cartoons and song lyricsPresents diverse perspectives, from elite policymakers to clergymen and novelistsFeatures documents from outside the United States, illustrating how people in other countries viewed America's role in the world

Winner of the Charles Taylor Prize for Literary Non-Fiction
Finalist for the Cundhill Prize in History
A richly detailed, profoundly engrossing story of how religion has influenced American foreign relations, told through the stories of the men and women--from presidents to preachers--who have plotted the country's course in the world.
Ever since John Winthrop argued that the Puritans' new home would be "a city upon a hill," Americans' role in the world has been shaped by their belief that God has something special in mind for them. But this is a story that historians have mostly ignored. Now, in the first authoritative work on the subject, Andrew Preston explores the major strains of religious fervor--liberal and conservative, pacifist and militant, internationalist and isolationist--that framed American thinking on international issues from the earliest colonial wars to the twenty-first century. He arrives at some startling conclusions, among them: Abraham Lincoln's use of religion in the Civil War became the model for subsequent wars of humanitarian intervention; nineteenth-century Protestant missionaries made up the first NGO to advance a global human rights agenda; religious liberty was the centerpiece of Franklin Roosevelt's strategy to bring the United States into World War II.
From George Washington to George W. Bush, from the Puritans to the present, from the colonial wars to the Cold War, religion has been one of America's most powerful sources of ideas about the wider world. When, just days after 9/11, George W. Bush described America as "a prayerful nation, a nation that prays to an almighty God for protection and for peace," or when Barack Obama spoke of balancing the "just war and the imperatives of a just peace" in his Nobel Peace Prize acceptance speech, they were echoing four hundred years of religious rhetoric. Preston traces this echo back to its source.
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"Sword of the Spirit, Shield of Faith" is an unprecedented achievement: no one has yet attempted such a bold synthesis of American history. It is also a remarkable work of balance and fair-mindedness about one of the most fraught subjects in America.