This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. Methods to assess mitochondrial function is of great interest to neuroscientists studying chronic forms of neurodegeneration, including Parkinson's, Alzheimer's, ALS, Huntington's and other triplet repeat diseases, but also to those working on acute conditions such as stroke and traumatic brain injury. This volume covers research methods on how to assess the life cycle of mitochondria including trafficking, fusion, fission, and degradation. Multiple perspectives on the complex and difficult problem of measurement of mitochondrial reactive oxygen species production with fluorescent indicators and techniques ranging in scope from measurements on isolated mitochondria to non-invasive imaging of metabolic function.

In this second of two new volumes covering mitochondria, methods developed to assess the number and function of nuclear-encoded proteins in the mitochondrion are presented. Chapters focus on the regulation of mitochondrial function and mitochondrial diseases, with a section emphasizing the mitochondrial defects associated with type 2 diabetes.
The critically acclaimed laboratory standard for 40 years, " Methods in Enzymology" is one of the most highly respected publications in the field of biochemistry. With more than 450 volumes published, each volume presents material that is relevant in today's labs -- truly an essential publication for researchers in all fields of life sciences.
New methods focusing on the examination of normal and abnormal mitochondrial function are presented in an easy-to-follow format by the researchers who developed them
Along with a companion volume covering topics including mitochondrial electron transport chain complexes and reactive oxygen species, provides a comprehensive overview of modern techniques in the study of mitochondrial malfunction
Provides a "one-stop shop" for tried and tested essential techniques, eliminating the need to wade through untested or unreliable methods

This edited collection attends to the locations of memory along and about the Indo-Pakistan and Indo-Bangladesh borders and the complex ways in which such memories are both allowed for and erased in the present. The collection is situated at the intersection of narratives connected to memory and commemoration in order to ask how memories have been formed and perpetuated across the imposition of these borders. It explores how national boundaries both silence memories and can be subverted in important ways, through consideration of physical sites and cultural practices on both sides of the India-Pakistan-Bangladesh borders that gesture towards that which has been lost - that is, the cultural whole that was the cultural regions of Punjab and Bengal before Partition, as well as broader cultural "wholes" across South Asia, across religious and linguistic lines - alongside forces that deny such connections. The chapters address issues of heritage and memory through specific case-studies on present-day memorial, museological and commemoration practices, through which sometimes competing memorial landscapes have been constructed, and show how memories of past traumas and histories become inscribed into diverse forms of cultural heritage (the built landscape, literature, film).

Anne Murphy offers a groundbreaking exploration of the material aspects of Sikh identity, showing how material objects, as well as holy sites, and texts, embody and represent the Sikh community as an evolving historical and social construction. Widening traditional scholarly emphasis on holy sites and texts alone to include consideration of iconic objects, such as garments and weaponry, Murphy moves further and examines the parallel relationships among sites, texts, and objects. She reveals that objects have played dramatically different roles across regimes-signifers of authority in one, mere possessions in another-and like Sikh texts, which have long been a resource for the construction of Sikh identity, material objects have served as a means of imagining and representing the past. Murphy's deft and nuanced study of the complex role objects have played and continue to play in Sikh history and memory will be a valuable resource to students and scholars of Sikh history and culture.

This volume brings together work by established and emerging scholars to consider the work and impact of Bhai Vir Singh. Bhai Vir Singh (1872-1957) was a major force in the shaping of modern Sikh and Punjabi culture, language, and politics in the undivided colonial Punjab, prior to the Partition of the province in 1947, and in the post-colonial state of India. The chapters in this book explore how he both reflected and shaped his time and context, and address some of the ongoing legacy of his work in the lives of contemporary Sikhs. The contributors analyze the varied genres, literary and historical, that were adopted and adapted by Bhai Vir Singh to foreground and enhance Sikh religiosity and identity. These include his novels, didactic pamphlets, journalistic writing, prefatory and exegetical work on spiritual and secular historical documents, and his poems and lyrics, among others. The book will be of particular interest to those working in Sikh studies, South Asian studies and post-colonial studies.

An intimate account of the eighteenth-century Bank of England that shows how a private institution became “a great engine of state” The eighteenth-century Bank of England was an institution that operated for the benefit of its shareholders—and yet came to be considered, as Adam Smith described it, “a great engine of state.” In Virtuous Bankers, Anne Murphy explores how this private organization became the guardian of the public credit upon which Britain’s economic and geopolitical power was based. Drawing on the voluminous and detailed minute books of a Committee of Inspection that examined the Bank’s workings in 1783–84, Murphy frames her account as “a day in the life” of the Bank of England, looking at a day’s worth of banking activities that ranged from the issuing of bank notes to the management of public funds. Murphy discusses the bank as a domestic environment, a working environment, and a space to be protected against theft, fire, and revolt. She offers new insights into the skills of the Bank’s clerks and the ways in which their work was organized, and she positions the Bank as part of the physical and cultural landscape of the City: an aggressive property developer, a vulnerable institution seeking to secure its buildings, and an enterprise necessarily accessible to the public. She considers the aesthetics of its headquarters—one of London’s finest buildings—and the messages of creditworthiness embedded in that architecture and in the very visible actions of the Bank’s clerks. Murphy’s uniquely intimate account shows how the eighteenth-century Bank was able to deliver a set of services that were essential to the state and commanded the confidence of the public.

Religious imaginary is a way of conceiving and structuring the world within the conceptual and imaginative traditions of the religious. Using religious imaginary as a reference, this book analyses temporal ideologies and expressions of historicity in South Asia in the early modern, pre-colonial and early colonial period. Chapters explore the multiple understandings of time and the past that informed the historical imagination in various kinds of literary representations, including historiographical and literary texts, hagiography, and religious canonical literature. The book addresses the contributing forces and comparative implications of the formation of religious and communitarian sensibilities as expressed through the imagination of the past, and suggests how these relate to each other within and across traditions in South Asia. By bringing diverse materials together, this book presents new commonalities and distinctions that inform a larger understanding of how religion and other cultural formations impinge on the concept of temporality, and the representation of it as history.

Religious imaginary is a way of conceiving and structuring the world within the conceptual and imaginative traditions of the religious. Using religious imaginary as a reference, this book analyses temporal ideologies and expressions of historicity in South Asia in the early modern, pre-colonial and early colonial period. Chapters explore the multiple understandings of time and the past that informed the historical imagination in various kinds of literary representations, including historiographical and literary texts, hagiography, and religious canonical literature. The book addresses the contributing forces and comparative implications of the formation of religious and communitarian sensibilities as expressed through the imagination of the past, and suggests how these relate to each other within and across traditions in South Asia. By bringing diverse materials together, this book presents new commonalities and distinctions that inform a larger understanding of how religion and other cultural formations impinge on the concept of temporality, and the representation of it as history.

In this poignant and meditative collection of short stories, Zubair Ahmad captures the lives and experiences of the people of the Punjab, a region divided between India and Pakistan. In an intimate narrative style, Ahmad writes a world that hovers between memory and imagination, home and abroad. The narrator follows the pull of his subconscious, shifting between past and present, recalling different eras of Lahore’s neighbourhoods and the communities that define them. These stories evoke the complex realities of post-colonial Pakistani Punjab. The contradictions and betrayals of this region’s history reverberate through the stories, evident in the characters, their circumstances, and sometimes their erasure. Skillfully translated from Punjabi by Anne Murphy, this collection is an essential contribution to the wider recognition of the Punjabi language and its literature.

This edited collection attends to the locations of memory along and about the Indo-Pakistan and Indo-Bangladesh borders and the complex ways in which such memories are both allowed for and erased in the present. The collection is situated at the intersection of narratives connected to memory and commemoration in order to ask how memories have been formed and perpetuated across the imposition of these borders. It explores how national boundaries both silence memories and can be subverted in important ways, through consideration of physical sites and cultural practices on both sides of the India-Pakistan-Bangladesh borders that gesture towards that which has been lost - that is, the cultural whole that was the cultural regions of Punjab and Bengal before Partition, as well as broader cultural "wholes" across South Asia, across religious and linguistic lines - alongside forces that deny such connections. The chapters address issues of heritage and memory through specific case-studies on present-day memorial, museological and commemoration practices, through which sometimes competing memorial landscapes have been constructed, and show how memories of past traumas and histories become inscribed into diverse forms of cultural heritage (the built landscape, literature, film).

Anne Murphy offers a groundbreaking exploration of the material aspects of Sikh identity, showing how material objects, as well as holy sites, and texts, embody and represent the Sikh community as an evolving historical and social construction. Widening traditional scholarly emphasis on holy sites and texts alone to include consideration of iconic objects, such as garments and weaponry, Murphy moves further and examines the parallel relationships among sites, texts, and objects. She reveals that objects have played dramatically different roles across regimes-signifers of authority in one, mere possessions in another-and like Sikh texts, which have long been a resource for the construction of Sikh identity, material objects have served as a means of imagining and representing the past. Murphy's deft and nuanced study of the complex role objects have played and continue to play in Sikh history and memory will be a valuable resource to students and scholars of Sikh history and culture.

A mysterious message carried by the wind encourages animals all across the land to head West for their protection and for a better future for their young. White Tail, Eagle, Newton, and others begin a long journey which takes them through many difficulties until they reach their Destiny, a small island in the middle of a vast ocean. Here, they settle peacefully, until a plane is spotted, threatening their new sense of security.

Tourism, with its wide-ranging impact, needs to be managed effectively - but how? This book advocates taking a business approach to tourism that encourages greater collaboration between stakeholders in the practical assessment of tourism options. The approach places key business management functions and stakeholders at the forefront of tourism initiatives. The business management functions of planning, organising, leadership and control are the filters through which tourism opportunities are viewed, while the stakeholder groups of customers, residents, industry and government set the agenda for appropriate tourism development. Tourist destinations must engage in realistic assessments of their abilities to meet the needs and expectations of tourism stakeholders and then act on these assessments so their goals and objectives can be achieved. A new model for bridging stakeholder gaps is presented as a template for how communities can understand and make the most of their tourism resources. The Bridging Tourism Gaps Model is a practical tool to help destinations focus on the important factors in developing and maintaining tourism as a beneficial and vital part of their communities. This book builds on the success of Tourism: A Community Approach and the subsequent tourism planning experiences of both authors to advance strategic planning in tourism.

Social care needs excellent leaders now more than ever. Effective leaders aim high, listen to what the service users want and need, inspire their staff and continually question what they are doing and why. This book draws together the latest research on fundamental leadership issues in social care, discussing collaborative leadership and the importance of place-based development, exploring the key disciplines of supervision, management and leadership and examining the purpose of a learning framework for social care. Comparative approaches are also provided by practitioners working outside of social work, placing leadership development in context across the public, private and voluntary sectors and presenting authoritative guidance from an international perspective. Leadership in Social Care will appeal to social care practitioners and service providers, academics, researchers and students who are passionate about making a difference for the people who use their services.