This new volume of Methods in Enzymology continues the legacy of
this premier serial with quality chapters authored by leaders in
the field. Methods to assess mitochondrial function is of great
interest to neuroscientists studying chronic forms of
neurodegeneration, including Parkinson's, Alzheimer's, ALS,
Huntington's and other triplet repeat diseases, but also to those
working on acute conditions such as stroke and traumatic brain
injury. This volume covers research methods on how to assess the
life cycle of mitochondria including trafficking, fusion, fission,
and degradation. Multiple perspectives on the complex and difficult
problem of measurement of mitochondrial reactive oxygen species
production with fluorescent indicators and techniques ranging in
scope from measurements on isolated mitochondria to non-invasive
imaging of metabolic function.
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