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The workshop "Genes and their products for tolerance to physical
stresses in plants" was held in Maratea, Italy, from 24-27
September 1995. As one of a series of activity launched by the
European Science Foundation (ESF) Network "Cell Stress Genes and
their Protein Products," the workshop was the only one entirely
devoted to research in the plant field. Around one hundred
participants from fourteen different countries enjoyed a lively
meeting in an atmosphere of sunny Mediterranean weather. A total of
twenty-eight speakers from universities and research institutes
were invited to present the most recent results of their research.
The workshop was divided into eight sessions, namely heat, cold,
salt, drought, oxidative stress, light stress, a conclusive session
for recommendations and a poster session. The results reported in
this workshop broadly showed the rapid progress made in the
understanding of the molecular mechanisms underlying basic aspects
of the plant response to environmental stresses.
Identification of differentially expressed genes is one of the
major challenges in molecular biology. Several techniques allow the
cloning of such sequences. However, methods such as RNA subtraction
or differential hybridization are time-consuming and require large
amounts of mRNA.
Recently, a new approach has successfully been developed:
Differential-Display Reverse Transcription-PCR (DDRT-PCR). This
technique has been proven to be highly effective in identifying
sequences that are differentially expressed in various cell types.
The most striking advantage is, however, that only nanograms of
total RNA are sufficient. Thus every mRNA species expressed in the
cell system can be investigated, even those present at very low
levels.
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