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The antiviral activity of plants Glyceriza glabra (roots),
Phyllanthus amblicus (Fruit), Eugenia jamolana (Leaves), and
Moringa oleifera (Leaves) were evaluated against Infectious bursal
disease virus (IBDv) in this study. Ethanolic extraction of these
plants was carried out by using Soxhlet apparatus. Four dilutions
of each extracts viz 100, 50, 25 and 12.5 g/ml were made. Vero
cells were infected by identified infectious bursal disease for
antiviral assay. Dilutions of these extracts were applied in
triplicate manner on Vero cells (infected by IBDv), that are
confluent in 96 well cell culture plates. The cytotoxic activity of
mentioned plant extracts was carried out by treating the cells by
the same dilutions as used in antiviral assay and incubated the
both 96 well cell culture plates for four days. After this
incubation, viability of cells was determined by MTT
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide]
colorimetric assay. Endpoint of these assays was measured in terms
of cell survival percentage. Results were compared for qualitative
variables using Chi-square technique and quantitative variables by
linear regression analysis.
Aseptic arthritis of the knee joint was induced in 12 healthy
donkeys, weighing 60 to 85kg by intra- articular injection of 0.3ml
of turpentine oil. The animals were randomly divided into 2 groups
A and B of 6 animals each. Group A served as a control whereas in
Group B anti-arthritic drug, diclofenac sodium (Diclocare 5%) was
administered intramuscularly at the dose rate of 2.5mg/kg body
weight daily for 5-6 days starting one day after the induction of
arthritis. In group B, the joint circumference became normal on 6th
day after induction of arthritis while in group A (control), the
joint circumference took 16 to 20 days to become normal. According
to this trial, diclofenac sodium provided an early return to normal
stance and weight bearing in treated group B, taking longer time in
control group A. No significant change in blood Haemoglobin, Total
erythrocyte count, Total leukocyte count, Erythrocyte sedimentation
rate and Packed cell volume was observed in control and treated
animals. It is concluded that diclofenac sodium is an effective
treatment for arthritis in donkeys.
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