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Understanding the functional roles of DNA modifications relies on
the accurate detection, quantification, and mapping of DNA
modifications. Methods for deciphering DNA modifications have
substantially improved over the last several years, which greatly
revolutionize the field of DNA modifications. In addition to DNA
cytosine methylation (5-methylcytosine, 5mC), the
best-characterized epigenetic modification, many new modifications
have been discovered to present in DNA in recent years. This book
provides a comprehensive overview of available techniques and
methods together with detailed step-by-step protocols for
experimental procedures required to successfully perform analysis
on various types of DNA modifications, including 5mC,
5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC),
5-carboxycytosine (5caC), 5-hydroxymethyluracil (5hmU),
5-formyluracil (5fU), N6-methyladenine (6mA),
-D-glucosyl-5-hydroxymethyluracil (base J) and
8-oxo-7,8-dihydroguanine (OG). This laboratory manual is a valuable
source for biochemists and molecular biologists from different
fields who wish to investigate DNA modifications.
Understanding the functional roles of DNA modifications relies on
the accurate detection, quantification, and mapping of DNA
modifications. Methods for deciphering DNA modifications have
substantially improved over the last several years, which greatly
revolutionize the field of DNA modifications. In addition to DNA
cytosine methylation (5-methylcytosine, 5mC), the
best-characterized epigenetic modification, many new modifications
have been discovered to present in DNA in recent years. This book
provides a comprehensive overview of available techniques and
methods together with detailed step-by-step protocols for
experimental procedures required to successfully perform analysis
on various types of DNA modifications, including 5mC,
5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC),
5-carboxycytosine (5caC), 5-hydroxymethyluracil (5hmU),
5-formyluracil (5fU), N6-methyladenine (6mA),
-D-glucosyl-5-hydroxymethyluracil (base J) and
8-oxo-7,8-dihydroguanine (OG). This laboratory manual is a valuable
source for biochemists and molecular biologists from different
fields who wish to investigate DNA modifications.
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