This first edition volume demystifies the complex topic of flow cytometry by providing detailed explanations and nearly 120 figures to help novice flow cytometry users learn and understand the bedrock principles necessary to perform basic flow cytometry experiments correctly. The book divides the topic of flow cytometry into easy to understand sections and covers topics such as the physics behind flow cytometry, flow cytometry lingo, designing flow cytometry experiments and choosing appropriate fluorochromes, compensation, sample preparation and controls and ways to assess cellular function using a variety of flow cytometry assays. Written as a series of chapters whose concepts sequentially build off one another, using the list of materials contained within each section along with the readily reproducible laboratory protocols and tips on troubleshooting that are included, readers should be able to reproduce the data figures presented throughout the book on their way to mastering sound basic flow cytometry techniques. Easy to understand and comprehensive, Flow Cytometry Basics for the Non-Expert will be a valuable resource to novice flow cytometry users as well as experts in other biomedical research fields who need to familiarize themselves with a basic understanding of how to perform flow cytometry and interpret flow cytometry data. This book is written for both scientists and non-scientists in academia, government, biotechnology, and medicine.

Burdened by famine, the plague, and economic hardship in the 1500s, the troubled citizens of Milan, mindful of their mortality, turned toward the veneration of the Virgin Mary and the creation of evangelical groups in her name. By 1594 the diversity of these lay religious organizations reflected in microcosm the varied expressions of Marian devotion in the Italian peninsula. Using archival documents, meditation and music books, and iconographical sources, Christine Getz examines the role of music in these Marian cults and confraternities in order to better understand the Church's efforts at using music to evangelize outside the confines of court and cathedral through its most popular saint. Getz reveals how the private music making within these cults, particularly among women, became the primary mode through which the Catholic Church propagated its ideals of femininity and motherhood.