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This first edition volume demystifies the complex topic of flow
cytometry by providing detailed explanations and nearly 120 figures
to help novice flow cytometry users learn and understand the
bedrock principles necessary to perform basic flow cytometry
experiments correctly. The book divides the topic of flow cytometry
into easy to understand sections and covers topics such as the
physics behind flow cytometry, flow cytometry lingo, designing flow
cytometry experiments and choosing appropriate fluorochromes,
compensation, sample preparation and controls and ways to assess
cellular function using a variety of flow cytometry assays. Written
as a series of chapters whose concepts sequentially build off one
another, using the list of materials contained within each section
along with the readily reproducible laboratory protocols and tips
on troubleshooting that are included, readers should be able to
reproduce the data figures presented throughout the book on their
way to mastering sound basic flow cytometry techniques. Easy to
understand and comprehensive, Flow Cytometry Basics for the
Non-Expert will be a valuable resource to novice flow cytometry
users as well as experts in other biomedical research fields who
need to familiarize themselves with a basic understanding of how to
perform flow cytometry and interpret flow cytometry data. This book
is written for both scientists and non-scientists in academia,
government, biotechnology, and medicine.
Burdened by famine, the plague, and economic hardship in the
1500s, the troubled citizens of Milan, mindful of their mortality,
turned toward the veneration of the Virgin Mary and the creation of
evangelical groups in her name. By 1594 the diversity of these lay
religious organizations reflected in microcosm the varied
expressions of Marian devotion in the Italian peninsula. Using
archival documents, meditation and music books, and iconographical
sources, Christine Getz examines the role of music in these Marian
cults and confraternities in order to better understand the
Church's efforts at using music to evangelize outside the confines
of court and cathedral through its most popular saint. Getz reveals
how the private music making within these cults, particularly among
women, became the primary mode through which the Catholic Church
propagated its ideals of femininity and motherhood.
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