This volume covers a wide array of topics that will aid researchers in the task of engineering complex biological systems. This book is divided into three parts: Part One discusses the discovery and identification of relevant biosynthetic pathways for engineering; Part Two looks at the development of genetic tools for manipulating enzymes, biosynthetic pathways, and whole genomes; and Part Three covers the characterization of engineered microbes using targeted and global systems biology tools, as well as in silico models. Chapters explore topics such as leveraging enzyme promiscuity to construct novel biosynthetic pathways; assembling combinatorial multigene pathways for rapid strain optimization; applying 'omics technologies for identifying bottlenecks; and engineering nontraditional host organisms like cyanobacterium and Yarrowia lipolytica. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and authoritative, Microbial Metabolic Engineering: Methods and Protocols is a valuable resource for researchers and scientists interested in engineering and optimizing microbes for a variety of biotechnological applications.

"Each chapter provides an overview of current knowledge on the topic in question, accompanied by an outline of advances in both scientific and applied fields. The chapters are written by twenty-five authors, all scientific experts in the subject discussed... In conclusion, Understanding the behaviour and improving the welfare of chickens is an expertly written, widely accessible book for all professionals in the field, which should provide increased understanding of chicken behaviour and welfare." (Animal Welfare - Universities Federation for Animal Welfare) With rising consumer concerns about the welfare of farm animals, such as chickens, there is a growing urgency for the livestock production sector to ensure that welfare standards are met throughout the supply chain, from breeding to slaughter. Understanding the behaviour and improving the welfare of chickens offers a comprehensive summary on the wealth of recent research completed on understanding chicken behaviour and discusses how best to use this rich body of knowledge to optimise welfare management of broilers and layers. This collection features expert insights into the use of wearable, video and acoustic technologies as a means of monitoring behaviour, as well as improving current welfare protocols. With its distinguished editor and team of leading experts in their fields, Understanding the behaviour and improving the welfare of chickens will be a standard text for university researchers in poultry and veterinary science as well as ethology. The book will also be an authoritative reference for government and other agencies responsible for the poultry sector and farm animal welfare, as well as companies involved in rearing chickens and processing poultry meat and eggs.

Chickens are by far the world's most widely farmed animal, kept for both meat and egg production. They are at the centre of many debates regarding housing and production systems, causing significant interest in what lies behind chicken behaviour. This accessible book covers sensory biology, behavioural development, preferences and aversions, social behaviour, learning and cognition, behavioural issues in different systems and solutions for behavioural problems. Authored by an authority on chicken ethology, it brings together the fields of animal behaviour, neuroscience, psychology and epidemiology to provide a comprehensive understanding of chicken behaviour and help improve the lives of farmed chickens around the world.

New Black author, Christine Copeland takes the reader on a journey through love and time in this poetry story. The turmoils and triumphs of love, loss, and knowledge of the self are all analyzed here. Contains various poems, psuedo-haikus, songs, and lyrical content.