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In this fully revised edition of an established classic, expert
researchers and clinicians describe in step-by-step detail updated
techniques for the isolation and growth of major primary cell
types, such as kidney proximal tubule cells, hepatocytes,
keratinocytes, and cardiomyocytes. The authors offer readily
reproducible new methods for the differentiation of embryonic stem
(ES) cells into various hematopoietic cell types, for fetal thymic
organ culture, and for the isolation and culture of specialized
cell types, such as mammary progenitor cells, skeletal muscle
myofibers, mesenchymal cells, neural stem cells, hematopoietic
cells, stromal cell lines, and endothelial cells. Additional
chapters describe new techniques (leukocyte rolling, isolation of
side-population cells, and scalable production of ES-derived cells)
and detail quality control methods for cell lines (detection and
elimination of mycoplasma, DNA fingerprinting, and cytogenetic
analysis).
At some point in their careers, virtually every scientist and
technician, as well as many medical professionals, regardless of
their area of specialization have a need to utilize cell culture
systems. Updating and significantly expanding upon the previous
editions, Basic Cell Culture Protocols, Fourth Edition provides the
novice cell culturist with sufficient information to perform the
basic techniques, to ensure the health and identity of their cell
lines, and to be able to isolate and culture specialized primary
cell types. The intent of this extensive volume is to generate a
valuable resource containing clear methodologies pertinent to
current areas of investigation, rather than attempting to educate
cell culturists on specific cell types or organ systems. It is
written in the highly successful Methods in Molecular Biology
trademark], chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Comprehensive and
up-to-date, Basic Cell Culture Protocols, Fourth Edition compiles
the essential techniques needed to approach this vital laboratory
activity with full success.
At some point in their careers, virtually every scientist and
technician, as well as many medical professionals, regardless of
their area of specialization have a need to utilize cell culture
systems. Updating and significantly expanding upon the previous
editions, Basic Cell Culture Protocols, Fourth Edition provides the
novice cell culturist with sufficient information to perform the
basic techniques, to ensure the health and identity of their cell
lines, and to be able to isolate and culture specialized primary
cell types. The intent of this extensive volume is to generate a
valuable resource containing clear methodologies pertinent to
current areas of investigation, rather than attempting to educate
cell culturists on specific cell types or organ systems. Written in
the highly successful Methods in Molecular Biology (TM), chapters
include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls. Comprehensive and up-to-date, Basic Cell
Culture Protocols, Fourth Edition compiles the essential techniques
needed to approach this vital laboratory activity with full
success.
In this fully revised edition of an established classic, expert
researchers and clinicians describe in step-by-step detail updated
techniques for the isolation and growth of major primary cell
types, such as kidney proximal tubule cells, hepatocytes,
keratinocytes, and cardiomyocytes. The authors offer readily
reproducible new methods for the differentiation of embryonic stem
(ES) cells into various hematopoietic cell types, for fetal thymic
organ culture, and for the isolation and culture of specialized
cell types, such as mammary progenitor cells, skeletal muscle
myofibers, mesenchymal cells, neural stem cells, hematopoietic
cells, stromal cell lines, and endothelial cells. Additional
chapters describe new techniques (leukocyte rolling, isolation of
side-population cells, and scalable production of ES-derived cells)
and detail quality control methods for cell lines (detection and
elimination of mycoplasma, DNA fingerprinting, and cytogenetic
analysis).
Child Maltreatment, Third Edition, by Cindy Miller-Perrin and Robin
Perrin, is a thoroughly updated new edition of the first textbook
for undergraduate students and beginning graduate students in this
field. The text is designed to provide a comprehensive introduction
to child maltreatment by disseminating current knowledge about the
various types of violence against children. By helping students
understand more fully the etiology, prevalence, treatment, policy
issues, and prevention of child maltreatment, the authors hope to
further our understanding of how to treat child maltreatment
victims and how to prevent future child maltreatment.
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