In this fully revised edition of an established classic, expert
researchers and clinicians describe in step-by-step detail updated
techniques for the isolation and growth of major primary cell
types, such as kidney proximal tubule cells, hepatocytes,
keratinocytes, and cardiomyocytes. The authors offer readily
reproducible new methods for the differentiation of embryonic stem
(ES) cells into various hematopoietic cell types, for fetal thymic
organ culture, and for the isolation and culture of specialized
cell types, such as mammary progenitor cells, skeletal muscle
myofibers, mesenchymal cells, neural stem cells, hematopoietic
cells, stromal cell lines, and endothelial cells. Additional
chapters describe new techniques (leukocyte rolling, isolation of
side-population cells, and scalable production of ES-derived cells)
and detail quality control methods for cell lines (detection and
elimination of mycoplasma, DNA fingerprinting, and cytogenetic
analysis).
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