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Adrenergic receptors are important modulators in the sympathetic c-
trol of various metabolic processes in the central and peripheral
nervous s- tems. These receptors are localized at multiple sites
throughout the central nervous system (CNS) and serve as important
regulators of CNS-mediated behavior and neural functions, including
mood, memory, neuroendocrine c- trol, and stimulation of autonomic
function. Adrenergic Receptor Protocols consists of 35 chapters
dealing with va- ous aspects of adrenergic receptor analyses,
including the use of genetic, RNA, protein expression,
transactivator, second messenger, immunocytochemical,
electrophysiological, transgenic, and in situ hybridization
approaches. This volume details the use of various methods to
examine the adrenergic receptor system, using aspects of the
genetic flow of information as a guide (DNA? RNA ? transactivator ?
protein expression ? second messenger analyses ? cellular analyses
? transgenic whole animal approaches). Adrenergic Receptor
Protocols displays step-by-step methods for s- cessful replication
of experimental procedures, and would be useful for both
experienced investigators and newcomers in the field, including
those beg- ning graduate study or undergoing postdoctoral training.
The Notes section contained in each chapter provides valuable
troubleshooting guides to help develop working protocols for your
laboratory. With Adrenergic Receptor Protocols, it has been my
intent to develop a comprehensive collection of modern molecular
methods for analyzing adrenergic receptors. I would like to thank
the many chapter authors for their contributions.
Viral Vectors for Gene Therapy: Methods and Protocols consists of
30 ch- ters detailing the use of herpes viruses, adenoviruses,
adeno-associated viruses, simple and complex retroviruses,
including lentiviruses, and other virus systems for vector
development and gene transfer. Chapter cont- butions provide
perspective in the use of viral vectors for applications in the
brain and in the central nervous system. Viral Vectors for Gene
Therapy: Methods and Protocols contains step-by-step methods for
successful rep- cation of experimental procedures, and should prove
useful for both experienced investigators and newcomers in the
field, including those beginning graduate study or undergoing
postdoctoral training. The "Notes" section contained in each
chapter provides valuable troublesho- ing guides to help develop
working protocols for your laboratory. With Viral Vectors for Gene
Therapy: Methods and Protocols, it has been my intent to develop a
comprehensive collection of modern molecular methods for the
construction, development, and use of viral vectors for gene
transfer and gene therapy. I would like to thank the many chapter
authors for their contributions. They are all experts in various
aspects of viral vectors, and I appreciate their efforts and hard
work in developing comprehensive chapters. As editor, it has been a
privilege to preview the development of Viral Vectors for Gene
Therapy: Methods and Protocols, and to acquire insight into the
various methodological approaches from the many different contri-
tors.
Viral Vectors for Gene Therapy: Methods and Protocols consists of
30 ch- ters detailing the use of herpes viruses, adenoviruses,
adeno-associated viruses, simple and complex retroviruses,
including lentiviruses, and other virus systems for vector
development and gene transfer. Chapter cont- butions provide
perspective in the use of viral vectors for applications in the
brain and in the central nervous system. Viral Vectors for Gene
Therapy: Methods and Protocols contains step-by-step methods for
successful rep- cation of experimental procedures, and should prove
useful for both experienced investigators and newcomers in the
field, including those beginning graduate study or undergoing
postdoctoral training. The "Notes" section contained in each
chapter provides valuable troublesho- ing guides to help develop
working protocols for your laboratory. With Viral Vectors for Gene
Therapy: Methods and Protocols, it has been my intent to develop a
comprehensive collection of modern molecular methods for the
construction, development, and use of viral vectors for gene
transfer and gene therapy. I would like to thank the many chapter
authors for their contributions. They are all experts in various
aspects of viral vectors, and I appreciate their efforts and hard
work in developing comprehensive chapters. As editor, it has been a
privilege to preview the development of Viral Vectors for Gene
Therapy: Methods and Protocols, and to acquire insight into the
various methodological approaches from the many different contri-
tors.
Adrenergic receptors are important modulators in the sympathetic c-
trol of various metabolic processes in the central and peripheral
nervous s- tems. These receptors are localized at multiple sites
throughout the central nervous system (CNS) and serve as important
regulators of CNS-mediated behavior and neural functions, including
mood, memory, neuroendocrine c- trol, and stimulation of autonomic
function. Adrenergic Receptor Protocols consists of 35 chapters
dealing with va- ous aspects of adrenergic receptor analyses,
including the use of genetic, RNA, protein expression,
transactivator, second messenger, immunocytochemical,
electrophysiological, transgenic, and in situ hybridization
approaches. This volume details the use of various methods to
examine the adrenergic receptor system, using aspects of the
genetic flow of information as a guide (DNA? RNA ? transactivator ?
protein expression ? second messenger analyses ? cellular analyses
? transgenic whole animal approaches). Adrenergic Receptor
Protocols displays step-by-step methods for s- cessful replication
of experimental procedures, and would be useful for both
experienced investigators and newcomers in the field, including
those beg- ning graduate study or undergoing postdoctoral training.
The Notes section contained in each chapter provides valuable
troubleshooting guides to help develop working protocols for your
laboratory. With Adrenergic Receptor Protocols, it has been my
intent to develop a comprehensive collection of modern molecular
methods for analyzing adrenergic receptors. I would like to thank
the many chapter authors for their contributions.
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