Human cell culture is not a new topic, but the development of new molecular techniques and reagents which can be used to investigate cell function and the responsible intracellular mechanisms make it a continuing requirement. This third edition of Human Cell Culture Protocols expands upon the previous editions with current, detailed protocols for the isolation and culture of a range of primary cells from human tissues. With new chapters on pancreatic cells needed for basic studies on the pathogenesis of diabetes and for their application for islet transplantation, the book also delves into protocols for hepatocytes, skin cells, lung cells, parathyroid cells, gastric cells, renal cells, adipocytes, ovarian cells, bone cells, vascular smooth muscle cells, vascular endothelial cells, regulatory T cells, blood mononuclear cells, as well as new techniques being applied to human cell culture, particularly the use of biocompatible scaffolds to grow cells, the in vitro use of laser microdissection to isolate cells from culture, and automated cell culture. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Human Cell Culture Protocols, Third Edition makes it possible for a worker with basic cell culture training, whether in the fields of cell biology, gene therapy, and cell transplantation, to prepare cell cultures of the specific cell type necessary to forward their vital research.

Victims of political persecution since 2000, Zimbabwe's whites have never overcome the problem of belonging. In North America and Australia, Europeans became the majority and "normal" partially through the genocide of native peoples. Settlers to Zimbabwe, however, only comprised a tiny minority. They monopolized the territory but struggled to assimilate culturally. Rather than integrating with African societies, many adopted a strategy of social escape. In this arresting and powerful study, David McDermott Hughes shows how they became emotionally and artistically invested in the non-human environment surrounding them. He traces how writers, artists, and farmers crafted a white identity focused on ecological conservation and how, emerging from state terror, some are now groping toward a whiteness of uncommon humanity and humility.

This edited volume presents a detailed portrait of couples living with mixed HIV status, where one partner is HIV-positive and the other negative. Readers will come to understand the various and complex ways in which these mixed-status, or serodiscordant couples build a life together within the shadow of HIV-related stigma. Spanning the globe, coverage explores serodiscordance as a negotiated practice and process, inseparable from the social context in which it is situated. The book shows how couples draw on diverse and sometimes contradictory cultural discourses of medicine, romance, and "normality" to make sense of and manage their mixed HIV status and any perceived risks, not uncommonly in ways that depart from prevailing HIV prevention messages. Throughout, compelling personal stories accompany the empirical research, sharing the firsthand experiences of men and women in serodiscordant relationships. Bringing together research from diverse disciplines and geographical regions, this book contributes important insights for future HIV health promotion as well as offers new knowledge to scholarship on the cultural intersections of illness and intimacy. It will appeal to a broad audience working across the fields of HIV, health, gender, sexuality, development, and human rights.

The reviews presented in this volume cover a huge range of cluster of galaxies topics. Readers will find the book essential reading on subjects such as the physics of the ICM gas, the internal cluster dynamics, and the detection of clusters using different observational techniques. The expert chapter authors also cover the huge advances being made in analytical or numerical modeling of clusters, weak and strong lensing effects, and the large scale structure as traced by clusters.

During the 19 years of her play-writing career, Aphra Behn had far more new plays staged than anyone else. This book is the first to examine all her theatrical work. It explains her often dominant place in the complex theatrical culture of Charles II's reign, her divided political sympathies, and her interests as a free-thinking intellectual. It also reveals her to be a brilliant theatrical practitioner who used the seen as richly and significantly as the spoken.

The Vita Christi, written by the abbess Isabel de Villena, is the only literary work in Catalan to bear the signature of a woman during the Middle Ages. It represents a fascinating re-evaluation of the role women played inthe life of Jesus Christ. The Life of Christ (Vita Christi), written by the abbess Isabel de Villena, is the only literary work to have been preserved in Catalan and to bear the signature of a woman during the Middle Ages. It was composed to provide spiritual direction for the nuns within the community of Poor Clares which Sor (i.e. Sister) Isabel oversaw at the Convent of the Holy Trinity in Valencia. The work was only able to emerge from obscurity by accident. In 1497 Queen Isabel of Castile, the wife of Ferdinand of Catalonia-Aragon, who had heard news of the book's existence, asked Sor Isabel's successor for a copy. The new Abbess, Sor Aldonca, responded by bringing the work to press. Queen Isabel's interest in Sor Isabel's book was understandable. The former abbess had been the daughter of the refined and restless Marquess of Villena, and was herself educated at Court, a milieu with which she maintained very positive relations throughout her life. As an abbess, what's more, she carried out important reforms at the convent and became a valued and respected figure within the dynamic cultural world of the Valencia of her day. Isabelde Villena's Vita Christi has often been interpreted as a response, delivered from the serenity of the cloister, to the misogyny and satire against the female gender emanating from certain books written at that time. Sor Isabel's work is a re-evaluation of the role women played in the life of Jesus Christ, a role at variance with the subsidiary one ascribed to them by the majority of commentators. Published in association with Editorial Barcino, Barcelona.

Global Women's Entrepreneurship Research responds to recent calls from academic researchers and policy analysts alike to pay greater attention to the diversity and heterogeneity among women entrepreneurs. Drawing together studies by 26 researchers affiliated with the DIANA International Research Network, this collection contributes to a richer and more robust understanding of the field. Part I: 'Diverse Settings' introduces research set in a range of contexts, from those rarely examined to those representing more familiar terrains. Part II: 'Diverse Questions' explores new questions and reframes old questions in fresh, innovative ways. Part III: 'Diverse Approaches' features studies with distinct methodological approaches that reflect and extend the rigour and creativity of research in this field. Together, the research assembled in this volume significantly advances knowledge about women's entrepreneurship around the world. While the book's primary audience is academic researchers and graduate students working in the areas of women's entrepreneurship, as well as entrepreneurship and family business more generally, it will also be of interest to scholars working in related research areas in the sociology of gender, work and organizations. Policy-makers in government and non-government agencies as well as profit and not-for-profit organizations that provide services to, or conduct research on, women entrepreneurs will also benefit greatly from the insights provided in this unique volume. Contributors: A.M. Bojica, S. Coleman, S.Y. Cooper, C. Diaz Garcia, C. Essers, M.R. Evald, N.C. Fairclough, M.M. Fuentes-Fuentes, P.G. Greene, D.M. Hechavarria i, K.D. Hughes, A.L. Humbert, A. Ingram, A. James, J.E. Jennings, P.D. Jennings, R. Justo, K. Klyver, S. Marlow, M. McAdam, S.L. Nielsen, M. Riebe, A. Robb, M. Sharifian, S. Terjesen, S.C. Zohir

The third title in "National Geographic Little Kids First Big Book" series, this book is for kids 4- to 8-years-old who LOVE dinos! The prehistoric world comes alive with dinosaurs small, big, giant, and gigantic, with stunning illustrations by Franco Tempesta--who illustrated "National Geographic Kids The Ultimate Dinopedia." Bursting with fun facts and age appropriate information, each spread features a different dinosaur, along with simple text in big type that is perfect for little kids. Young dino fans will love the interactivity included in every chapter, and parents will appreciate tips to help carry readers' experience beyond the page.

Approx.562 pages

Japanese corporate governance and managerial practice is at a critical juncture. At the start of the decade pressures mounted for Japan to move to a shareholder-value driven, "Anglo-American" system of corporate governance. Subsequent changes, however, may be seen as an adjustment and renewal of the post-war model of the Japanese firm. In adapting to global corporate governance standards, Japanese managers have also been reshaping them according to their own agenda of reform and restructuring of decision-making processes.
The board's role is seen in terms of strategic planning rather than monitoring, and external directors are viewed as advisers, not as representatives of the shareholders. Managers have adopted a variety of defences against hostile takeovers, including poison pills in some cases. Although shareholder influence is more extensive than it was, central aspects of the Japanese "community firm" remain in place. The commitment to stable or "lifetime" employment for a core of employees, although coming under severe pressure, is still an important point of reference for Japanese management.
Corporate Governance and Managerial Reform in Japan is based on detailed and intensive field work in large Japanese companies and interviews with investors, civil servants, and policy makers in the period following the adoption of significant corporate law reforms in the early 2000s up to the months just before the global financial crisis of 2008. The Japanese experience suggests that there are limits to the global convergence of company law systems, and that the widespread association of Anglo-American practices with the "modernization" of corporate governance has been misplaced. This conclusion is unlikely to be altered--it may be reinforced--by reactions to the financial crisis.

Numerous ancient texts describe human sacrifices and other forms of ritual killing: in 480 BC Themistocles sacrifices three Persian captives to Dionysus; human scapegoats called pharmakoi are expelled yearly from Greek cities, and according to some authors they are killed; Locrin girls are hunted down and slain by the Trojans; on Mt Lykaion children are sacrificed and consumed by the worshippers; and many other texts report human sacrifices performed regularly in the cult of the gods or during emergencies such as war and plague. Archaeologists have frequently proposed human sacrifice as an explanation for their discoveries: from Minoan Crete children's bones with knife-cut marks, the skeleton of a youth lying on a platform with a bronze blade resting on his chest, skeletons, sometimes bound, in the dromoi of Mycenaean and Cypriot chamber tombs; and dual man-woman burials, where it is suggested that the woman was slain or took her own life at the man's funeral. If the archaeologists' interpretations and the claims in the ancient sources are accepted, they present a bloody and violent picture of the religious life of the ancient Greeks, from the Bronze Age well into historical times. But the author expresses caution. In many cases alternative, if less sensational, explanations of the archaeological are possible; and it can often be shown that human sacrifices in the literary texts are mythical or that late authors confused mythical details with actual practices.Whether the evidence is accepted or not, this study offers a fascinating glimpse into the religious thought of the ancient Greeks and into changing modern conceptions of their religious behaviour.

A scholarly and engaging study, this history of Swaziland, by an author who spent many years in the kingdom, presents a vivid account of the interplay of politics and personalities along the passage to post-colonial independence. From the early stages of Swazi occupation of the present-day kingdom to the accession of Sobhuza II as king in 1921, this book traces problems in consolidating leadership under the Dlamini chieftaincy and examines the infuence of Boer and British settlers, and of mining and commercial interests, on Swazi culture and governance. It recounts the story of a thriving small nation that sought to maintain traditional customs and institutions in the face of a powerful European presence.

Each of the sixteen chapters concentrates on an aspect of political history that has influenced the character of the present-day kingdom, and much of the material, especially after 1900, has not been utilized in previous studies. The introduction looks at Swazi experience in a contemporary context, evaluating historic forces that have made for stability in a rapidly changing world. Other sections detail the Swazi reaction to European-controlled neighboring states (the Transvaal, Natal, and Mozambique), the tensions introduced by successive Boer and British policies, the Swazi detachment during two external wars (1899-1902 and 1914-1918), and widespread concerns about colonialism and self-governance following World War I.

Numerous ancient texts describe human sacrifices and other forms of ritual killing: in 480 BC Themistocles sacrifices three Persian captives to Dionysus; human scapegoats called "pharmakoi" are expelled yearly from Greek cities and, according to some authors, they are killed. Many texts speak of human sacrifices performed regularly in the cult of the gods or during emergencies such as war and plague. Archaeologists have also frequently proposed human sacrifice as an explanation for their discoveries. If the archaeologists' interpretations and the claims in the ancient sources are accepted, they present a bloody and violent picture of the religious life of the Ancient Greeks, from the Bronze Age well into historical times. The author of this study, however, counsels caution. Following an examination of the written and archaeological evidence of ritual human killings, he argues that many of the archaeological findings are uncertain, and in many cases alternative explanations are possible.

Ronald Dore's enquiring mind, rigorous reasoning and comparative methodology have greatly enhanced our understanding of Japan. His insights from Japan have been deployed to generate fresh perspectives on Britain and other industrialized and developing countries. This careful selection of writings reflects his underlying concern with what light the study of Japan sheds on theoretical generalizations about how societies evolve and how economies work. Social Evolution, Economic Development and Culture brings together Ronald Dore's key writings for the first time, making his work accessible across a wide range of social science disciplines. It produces a distinctive perspective with four interlinking themes - technology-driven social evolution, late development, culture and polemics. These are highly topical in the current context of rapid technological innovation and socio-economic change, globalization and accompanying policy choices. The book provides a rich empirical and conceptual source for those interested in technology, socio-economic evolution and culture, and the ways in which they interact. Researchers, teachers and students in the fields of evolutionary economics, economic development, comparative education, institutional economics, political economy and economic and classical sociology (as well as Japanese studies) will find this volume invaluable reading.

This beautiful book is the latest addition to the "National Geographic Little Kids First Big Book" series. These colorful pages will introduce young children to the wonders of space, with colorful illustrations by David Aguilar and simple text that is perfect for beginning readers or for reading aloud. The book will explain basic concepts of space, beginning with what is most familiar to kids and expanding out into universe.
Chapters include:
- Chapter 1 focuses on the Earth, moon, and sun.
- Chapter 2 introduces kids to the other planets in our solar system.
- Chapter 3 explains other objects in our solar system, such as dwarf planets, comets, and asteroid belts.
- Chapter 4 voyages even farther afield, touching on concepts such as the universe, the Milky Way, stars, galaxies, and black holes.
- The last chapter delves into space exploration: humans on the moon, spaceships, the International Space Station, etc.
Releases simultaneously in Reinforced Library Binding: 978-1-4263-1015-7, $23.90/$26.95 Can

Stuck in a rut? Know what you want but don't know how to get it? Feel like life is passing you by? Sick of getting mediocre results? Then enter the wonderful world of Liquid Thinking...

A practical, jargon-free and easily accessible self-help book drawing on a diverse range of experiences and containing digestible lessons and exercises used by sports captains, charity leaders and business leaders. It is the only self-help book which has ever been endorsed by Sir Richard Branson, Angelo Dundee, Muhammad Ali, and Jonny Wilkinson.

It is a brave man who starts his book on self development by quoting Jerry Springer and discussing the literary merits of the "Joy of Sex"; however, this is Damian Hughes to a tee. Combining his own experiences as a Manchester United football coach, HR Director and youth club leader with exclusive insights from Sir Richard Branson, Angelo Dundee, Muhammad Ali and Jonny Wilkinson, Hughes will help you to step forward to achieve your own special hopes, dreams and ambition.

The books have been credited with helping people build their own houses, fight cancer and run marathons, so come on and be a fellow Liquid Thinker

A selection from the works of the Catalan Franciscan, Francesc Eiximenis. Francesc Eiximenis is an outstanding figure in the ranks of the mendicant orders who, in the late Middle Ages, strove to educate the lower echelons of society. Born in Gerona, around 1330, probably to a comfortable middle-class family, Eiximenis entered the Franciscan order at a very early age, studied in Oxford, and probably also in Paris, and obtained the degree of master of theology in Toulouse. Later he combined teaching with the composing of his works. Among these stands out the monumental and widely known Lo Crestia (The Christian), in which Eiximenis aimed to include all contemporary university knowledge, adapted for a lay public whose basic level of instruction was far below that of clerics. The same didactic purpose is seen in the Libro de los angeles (Book of the Angels), the Libro de las mujeres (Book of the Women) and the Vita Christi. Eiximenis, by then bishop of Elna, died in 1409. Among the many themes that recur in his extensive literary production, this anthology concentrates on his ideas on the transmission of knowledge, on education and on culture. The introduction and selection of texts is by David Guixeras and Xavier Renedo. ROBERT D. HUGHES is a translator and researcher with particular expertise in the fields of fine art, the history of ideas and Catalan culture. Published in association with Editorial Barcino

Human cell culture is not a new topic, but the development of new molecular techniques and reagents which can be used to investigate cell function and the responsible intracellular mechanisms make it a continuing requirement. This third edition of Human Cell Culture Protocols expands upon the previous editions with current, detailed protocols for the isolation and culture of a range of primary cells from human tissues. With new chapters on pancreatic cells needed for basic studies on the pathogenesis of diabetes and for their application for islet transplantation, the book also delves into protocols for hepatocytes, skin cells, lung cells, parathyroid cells, gastric cells, renal cells, adipocytes, ovarian cells, bone cells, vascular smooth muscle cells, vascular endothelial cells, regulatory T cells, blood mononuclear cells, as well as new techniques being applied to human cell culture, particularly the use of biocompatible scaffolds to grow cells, the in vitro use of laser microdissection to isolate cells from culture, and automated cell culture. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Human Cell Culture Protocols, Third Edition makes it possible for a worker with basic cell culture training, whether in the fields of cell biology, gene therapy, and cell transplantation, to prepare cell cultures of the specific cell type necessary to forward their vital research.

Are entrepreneurs essentially the same everywhere? Are the processes of entrepreneurship similar? Or are they shaped by their environments? If so, how?
We know a lot about national differences in management practices, corporate governance, and even innovation systems, but we know surprisingly little about national differences in entrepreneurship. Comparative Entrepreneurship compares processes of entrepreneurship in the UK and Japan, countries associated with liberal market economies and coordinated market economies respectively.
Focusing on high tech manufacturing it identifies basic similarities and key differences. Similarities are found in approaches to opportunity and business creation, which are strikingly different from recent policy emphases in the UK and Japan, inspired by Silicon Valley (hence the entrepreneurs live in Wthe shadow of Silicon Valley"). Differences--in the backgrounds of entrepreneurs, founding teams, attitudes to growth and risk, innovation, competitive advantages, HRM emphases, and inter-firm collaborations--are summed up by the concepts of "project entrepreneurship" and "lifework entrepreneurship." These are closely related to the respective environments, especially the nature of markets in both countries. They also embody different time orientations, with implications for financing and governance.
This study brings insights from entrepreneurship to comparative institutions and varieties of capitalism, and vice versa, and draws on two surveys and 25 case interviews in both the UK and Japan. It concludes with a discussion of dilemmas for entrepreneurship policy in the UK, Japan, and other countries.

This, the first of two volumes devoted to probability theory in physics, physical chemistry, and engineering, provides an introduction to the problem of the random walk and its applications. In its simplest form, the random walk describes the motion of an idealized drunkard and is a discrete analogue of the diffusion process.

A thorough account is given of the theory of random walks on discrete spaces (lattices or networks) and in continuous spaces, including processed with random waiting times between steps. Applications discussed include dielectric relaxation, charge transport in the xerographic process, turbulent dispersion, diffusion through a medium with traps, laser speckle and the conformations of polymers in dilute solution. Prior knowledge of probability theory would be helpful, but not assumed. An extensive bibilography concludes the book.

The first major work of literature written in Catalan and arguably the first European novel. The Romance of Evast and Blaquerna is a novel in which Ramon Llull rebelled against secular literature of chivalry and the court in general, which was so successful in his time. Written around 1283, it tells the fictional biography of Blaquerna, a virtuous young man who aspires to be a hermit, not a knight, but who on encountering the disorder of the world is obliged to remain in it. In his reform efforts, Blaquerna passes through several estates before being elected Holy Father in Rome, allowing him to intervene in all Christendom and work decidedly in favor of the conversion of infidels. With everyone on the path to salvation, he may eventually retire and devote himself tothe contemplative life. In this new situation, Blaquerna wrote the Book of the Lover and the Beloved, one of the top works of mystical literature of all time. Published in association with Editorial Barcino. Ramon Llull (1232-1316) was a mystic, missionary, philosopher and author of narrative and poetry. He is credited with writing the first major work of Catalan literature. Robert D. Hughes is a specialist in medieval Catalan literature and a widely published translator.

The reviews presented in this volume cover a huge range of cluster of galaxies topics. Readers will find the book essential reading on subjects such as the physics of the ICM gas, the internal cluster dynamics, and the detection of clusters using different observational techniques. The expert chapter authors also cover the huge advances being made in analytical or numerical modeling of clusters, weak and strong lensing effects, and the large scale structure as traced by clusters.