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High Throughput Bioanalytical Sample Preparation: Methods and
Automation Strategies is an authoritative reference on the current
state-of-the-art in sample preparation techniques for bioanalysis.
This book focuses on high throughput (rapid productivity)
techniques and describes exactly how to perform and automate these
methodologies, including useful strategies for method development
and optimization. A thorough review of the literature is included
within each of these chapters describing high throughput sample
preparation techniques: protein removal by precipitation;
equilibrium dialysis and ultrafiltration; liquid-liquid extraction;
solid-phase extraction; and various on-line techniques.
The text begins with an introductory overview of the role of
bioanalysis in pharmaceutical drug development. Fundamental
understanding of the strategies for sample preparation is
reinforced next, along with essential concepts in extraction
chemistry. Several chapters introduce and discuss microplates,
accessory products and automation devices. Particular strategies
for efficient use of automation within a bioanalytical laboratory
are also presented. The subject material then reviews protein
precipitation, liquid-liquid extraction, solid-phase extraction and
various on-line sample preparation approaches. The book concludes
with information on recent advances in sample preparation, such as
solid-phase extraction in a card format and higher density
extraction plates.
Important objectives that can be accomplished when the strategies
presented in this book are followed include: improved efficiency in
moving discovery compounds to preclinical status with robust
analytical methods; return on investment in automation for sample
preparation; and improved knowledge and expertise of laboratory
staff.
.Shows the reader exactly how to perform modern bioanalytical
sample preparation techniques, complete with detailed
strategies
.Thorough literature review and summary of published
information
.Detailed discussion and examples of the method development
process"
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