Brucella is a genus of Gram-negative, facultative, intracellular bacteria that are highly pathogenic for a variety of mammals, including humans. Recently the WHO cited brucellosis to be the world's most widespread zoonosis. An important feature of the pathogenicity of these organisms is their ability to survive and replicate within the host macrophages. However the mechanism for this is unclear. In addition, none of the classical bacterial virulence factors found in other bacterial pathogens have been found in the genomes of the forty Brucella species and biovars analysed to date. Nevertheless the application of systems biology approaches in recent years has transformed research, permitting fascinating new insights into Brucella molecular biology and genomics. Written by highly acclaimed Brucella scientists, this book comprehensively reviews the most important advances in the field. Opening chapters focus on genetic diversity within Brucella, covering both classical and new species. Pa

"Host-Bacteria Interactions: Methods and Protocols" details cutting edge protocols that cover aspects of the investigation of host bacteria interactions using mammalian and novel non mammalian infection models, cell biology, OMICS and bacterial genetics. Chapters focus on techniques that can be used to investigate different aspects of the physiopathology of bacterial infections, from the whole animal to tissue, cellular and molecular level. Written in the highly successful "Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, "Host-Bacteria Interactions: Methods and Protocols "provide researchers with a comprehensive account of the practical steps necessary for carrying out each protocol successfully.

Host-Bacteria Interactions: Methods and Protocols details cutting edge protocols that cover aspects of the investigation of host bacteria interactions using mammalian and novel non mammalian infection models, cell biology, OMICS and bacterial genetics. Chapters focus on techniques that can be used to investigate different aspects of the physiopathology of bacterial infections, from the whole animal to tissue, cellular and molecular level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Host-Bacteria Interactions: Methods and Protocols provide researchers with a comprehensive account of the practical steps necessary for carrying out each protocol successfully.