0
Your cart

Your cart is empty

Browse All Departments
  • All Departments
Price
  • R2,500 - R5,000 (2)
  • R5,000 - R10,000 (2)
  • -
Status
Brand

Showing 1 - 4 of 4 matches in All Departments

Low and Intermediate Energy Kaon-Nucleon Physics - Proceedings of the Workshop held at the Institute of Physics of the... Low and Intermediate Energy Kaon-Nucleon Physics - Proceedings of the Workshop held at the Institute of Physics of the University of Rome, March 24-28, 1980 (Hardcover, 1981 ed.)
E. Ferrari, G. Violini
R4,463 Discovery Miles 44 630 Ships in 12 - 17 working days

Proceedings of the Workshop held at the University of Rome, March 24-28, 1980

Molecular Basis of Neurological Disorders and Their Treatment (Hardcover, 1991 ed.): J.W. Gorrod, A. Albano, E. Ferrari, S. Papa Molecular Basis of Neurological Disorders and Their Treatment (Hardcover, 1991 ed.)
J.W. Gorrod, A. Albano, E. Ferrari, S. Papa
R5,631 Discovery Miles 56 310 Ships in 10 - 15 working days

cytochemical techniques (ICC) which provide a useful adjunct to investigations by immunoblotting. A particular advantage of a cytochemical approach to the investiga tion of mitochondrial disorders is that it allows the mosaic distribution of certain of these defects to be detected, whereas the tissue homogeniza tion involved in conventional enzyme assays or immunoblotting precludes this. A further advantage of MEA or ICC is that only small amounts of tissue are needed, which is important since many of the affected patients are infants or small children. The main aim of this communica tion is to outline ways in which these techniques can be used in the diagnosis and further investigation of mitochondrial disorders. Reference will be made not only to those situations in which MEA and ICC offer advantages over standard enzyme asays and immunoblotting but also to contexts in which the reverse applies. 4. 2 MATERIALS AND METHODS Muscle biopsies for cytochemical investigation were snap-frozen using isopentane cooled to - 150 DegreesC in liquid nitrogen. Samples were stored in heat-sealed polythene packets in the vapour phase of liquid nitrogen containers. 4. 2. 1 Microphotometric enzyme assays Frozen sections 8 Jlm thick were cut using a Reichert-J ung Frigocut cryostat microtome equipped with motor-driven cutting action to maintain maximal reproducibility of section thickness. Sections were picked up on microscope slides and air-dried for 15 min at room temperature.

Molecular Basis of Neurological Disorders and Their Treatment (Paperback, Softcover reprint of the original 1st ed. 1991): J.W.... Molecular Basis of Neurological Disorders and Their Treatment (Paperback, Softcover reprint of the original 1st ed. 1991)
J.W. Gorrod, A. Albano, E. Ferrari, S. Papa
R5,445 Discovery Miles 54 450 Ships in 10 - 15 working days

cytochemical techniques (ICC) which provide a useful adjunct to investigations by immunoblotting. A particular advantage of a cytochemical approach to the investiga tion of mitochondrial disorders is that it allows the mosaic distribution of certain of these defects to be detected, whereas the tissue homogeniza tion involved in conventional enzyme assays or immunoblotting precludes this. A further advantage of MEA or ICC is that only small amounts of tissue are needed, which is important since many of the affected patients are infants or small children. The main aim of this communica tion is to outline ways in which these techniques can be used in the diagnosis and further investigation of mitochondrial disorders. Reference will be made not only to those situations in which MEA and ICC offer advantages over standard enzyme asays and immunoblotting but also to contexts in which the reverse applies. 4. 2 MATERIALS AND METHODS Muscle biopsies for cytochemical investigation were snap-frozen using isopentane cooled to - 150 DegreesC in liquid nitrogen. Samples were stored in heat-sealed polythene packets in the vapour phase of liquid nitrogen containers. 4. 2. 1 Microphotometric enzyme assays Frozen sections 8 Jlm thick were cut using a Reichert-J ung Frigocut cryostat microtome equipped with motor-driven cutting action to maintain maximal reproducibility of section thickness. Sections were picked up on microscope slides and air-dried for 15 min at room temperature.

Low and Intermediate Energy Kaon-Nucleon Physics - Proceedings of the Workshop held at the Institute of Physics of the... Low and Intermediate Energy Kaon-Nucleon Physics - Proceedings of the Workshop held at the Institute of Physics of the University of Rome, March 24-28, 1980 (Paperback, Softcover reprint of the original 1st ed. 1981)
E. Ferrari, G. Violini
R4,272 Discovery Miles 42 720 Ships in 10 - 15 working days

Proceedings of the Workshop held at the University of Rome, March 24-28, 1980

Free Delivery
Pinterest Twitter Facebook Google+
You may like...
Loot
Nadine Gordimer Paperback  (2)
R383 R318 Discovery Miles 3 180
Loot
Nadine Gordimer Paperback  (2)
R383 R318 Discovery Miles 3 180
3 Layer Fabric Face Mask (Blue)
R15 Discovery Miles 150
Multi-Functional Bamboo Standing Laptop…
 (1)
R995 R399 Discovery Miles 3 990
Loot
Nadine Gordimer Paperback  (2)
R383 R318 Discovery Miles 3 180
Lucky Define - Plastic 3 Head…
R390 Discovery Miles 3 900
Canon 445 Original Ink Cartridge (Black)
R700 R335 Discovery Miles 3 350
Loot
Nadine Gordimer Paperback  (2)
R383 R318 Discovery Miles 3 180
Casio LW-200-7AV Watch with 10-Year…
R999 R884 Discovery Miles 8 840
The Creator
John David Washington, Gemma Chan, … DVD R312 Discovery Miles 3 120

 

Partners