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Molecular Basis of Neurological Disorders and Their Treatment (Paperback, Softcover reprint of the original 1st ed. 1991)
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Discovery Miles 54 450
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Molecular Basis of Neurological Disorders and Their Treatment (Paperback, Softcover reprint of the original 1st ed. 1991)
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cytochemical techniques (ICC) which provide a useful adjunct to
investigations by immunoblotting. A particular advantage of a
cytochemical approach to the investiga tion of mitochondrial
disorders is that it allows the mosaic distribution of certain of
these defects to be detected, whereas the tissue homogeniza tion
involved in conventional enzyme assays or immunoblotting precludes
this. A further advantage of MEA or ICC is that only small amounts
of tissue are needed, which is important since many of the affected
patients are infants or small children. The main aim of this
communica tion is to outline ways in which these techniques can be
used in the diagnosis and further investigation of mitochondrial
disorders. Reference will be made not only to those situations in
which MEA and ICC offer advantages over standard enzyme asays and
immunoblotting but also to contexts in which the reverse applies.
4. 2 MATERIALS AND METHODS Muscle biopsies for cytochemical
investigation were snap-frozen using isopentane cooled to - 150
DegreesC in liquid nitrogen. Samples were stored in heat-sealed
polythene packets in the vapour phase of liquid nitrogen
containers. 4. 2. 1 Microphotometric enzyme assays Frozen sections
8 Jlm thick were cut using a Reichert-J ung Frigocut cryostat
microtome equipped with motor-driven cutting action to maintain
maximal reproducibility of section thickness. Sections were picked
up on microscope slides and air-dried for 15 min at room
temperature.
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