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This book addresses the complexity of mixed language classroom
learning environments in which heritage learners (HL) and second
language (L2) learners are concurrently exposed to language
learning in the same physical space. Heritage speakers, defined
widely as those exposed to the target language at home from an
early age, tend to display higher oral proficiency and increased
intercultural proficiency but lesser metalinguistic and grammatical
awareness than L2 learners. The theoretical and pedagogical
challenges of engaging both types of learners simultaneously
without polarizing the classroom community dictates the need for
well-defined, differentiated learning strategies; in response this
book offers best practices and reproducible pedagogical initiatives
and methodologies for different levels of instruction. The chapters
address themes including translanguaging, linguistic identity,
metalinguistic awareness and intercultural competence, with
contributions from Europe, Africa and the United States.
This book addresses the complexity of mixed language classroom
learning environments in which heritage learners (HL) and second
language (L2) learners are concurrently exposed to language
learning in the same physical space. Heritage speakers, defined
widely as those exposed to the target language at home from an
early age, tend to display higher oral proficiency and increased
intercultural proficiency but lesser metalinguistic and grammatical
awareness than L2 learners. The theoretical and pedagogical
challenges of engaging both types of learners simultaneously
without polarizing the classroom community dictates the need for
well-defined, differentiated learning strategies; in response this
book offers best practices and reproducible pedagogical initiatives
and methodologies for different levels of instruction. The chapters
address themes including translanguaging, linguistic identity,
metalinguistic awareness and intercultural competence, with
contributions from Europe, Africa and the United States.
This volume serves to aid researchers working in the recombinant
protein production field by describing a wide number of protocols
and examples. Chapters describe recombinant protein production in
different expression systems, prokaryotic and eukaryotic expression
systems, purification protocols, characterization of insoluble
proteins and a general overview of interesting applications of
insoluble proteins. Written in the format of the highly successful
Methods in Molecular Biology series, each chapter includes an
introduction to the topic, lists necessary materials and reagents,
includes tips on troubleshooting and known pitfalls, and
step-by-step, readily reproducible protocols. Authoritative and
cutting-edge, Insoluble Proteins: Methods and Protocols, Second
Edition aims to be a useful practical guide to researches to help
further their study in this field.
With insolubility proving to be one of the most crippling
bottlenecks in the protein production and purification process,
this volume serves to aid researchers working in the recombinant
protein production field by describing a wide number of protocols
and examples. Insoluble Proteins: Methods and Protocols includes
chapters that describe not only the recombinant protein production
in different expression systems but also different purification and
characterization methods to finally obtain these
difficult-to-obtain proteins. Beginning with protein production
methods using both prokaryotic and eukaryotic expression systems,
the book continues with purification protocols using insoluble
proteins, the characterization of insoluble proteins, as well as a
general overview of interesting applications of insoluble proteins.
Written in the highly successful Methods in Molecular Biology
series format, chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Comprehensive and
practical, Insoluble Proteins: Methods and Protocols aims to
provide the scientific community with detailed and reliable
state-of-the-art protocols that are used in order to successfully
produce and purify recombinant proteins prone to aggregate.
Walking machines have potential advantages over traditional
vehicles, and they have already succeeded in carrying out many
tasks that wheeled or tracked robots cannot handle. Nevertheless,
their use in industry and services is currently limited in scope.
Quadrupedal Locomotion: An Introduction to the Control of
Four-legged Robots brings together some of the methods and
techniques in this emerging field that have recently been developed
in an effort to deal with the problems that currently prevent
legged robots being more widely used for real applications.
Quadrupedal Locomotion: An Introduction to the Control of
Four-legged Robots illustrates the appropriate algorithms and
methods through a discussion of simulation and experiments that
have been tested on a real machine, the SILO4 walking robot. Data
from the experiments can be found on-line at http:
//www.iai.csic.es/users/silo4/. This book is divided into two
parts: the first part, Walking Measurements and Algorithms,
introduces the historical development of quadrupeds, their
advantages/disadvantages and potential uses, and the trade-off
between quadrupeds and hexapods. The second part, Control
Techniques, concentrates on general techniques that have been
specifically applied to legged robots, including kinematic and
dynamic models, soft computing techniques to increase speed,
virtual sensors that help reduce the electronic burden of the
machine, and software simulators to study and test certain robot
properties.
As the first book to focus specifically on quadrupeds,
Quadrupedal Locomotion: An Introduction to the Control of
Four-legged Robots will be suitable for researchers, postgraduates
and senior undergraduates inthe field of robotics as well as
engineers working in industry.
This work focuses on rural community versions of Spanish Early
Modern Theatre and deals with cultural heritage and the
contemporary impact of Golden Age theatre on local rural
communities. To this end, I examine the burgeoning of annual rural
Golden Age theatre festivals that generate site-centered,
non-professional productions of the plays, and revisit the conflict
between tradition and innovation, between popular and high culture
between authority of literary heritage and the people's right to
the canon. The selection of Early Modern plays set in actual
Spanish communities-Fuenteovejuna, El Alcalde de Zalamea, Numancia
and Los tres blasones de Espana-renders an overview of the effect
of these important works on their respective communities and
focuses on the theatrical festivals as peripheral, subaltern,
hybrid cultural phenomena. I take into consideration not only
traditional and significant studies on these four renowned plays,
but recent theories on staging, performance and popular reception
and agency. The research involved crosses traditional disciplinary
boundaries between literature, history, geography, and politics by
centering on the appropriation and re-examination of a past that is
continuously revised through contemporary performance, and which is
adjusted to fit the needs and desires of the context in which it is
interpreted. This diachronic approach allows for a new perspective
on contemporary performances which question cultural politics,
redefine tradition and transcend geo-political boundaries.
Biomaterials for Skin Repair and Regeneration examines a range of
materials and technologies used for regenerating or repairing skin.
With a strong focus on biomaterials and scaffolds, the book also
examines the testing and evaluation pathway for human clinical
trials. Beginning by introducing the fundamentals on skin tissue,
the book goes on to describe contemporary technology used in skin
repair as well as currently available biomaterials suitable for
skin tissue repair and regeneration. Skin tissue engineering and
the ideal requirements to take into account when developing skin
biomaterials are discussed, followed by information on the
individual materials used for skin repair and regeneration. As
evaluation of biomaterials in animal models is mandatory before
proceeding into human clinical trials, the book also examines the
different animal models available. With a strong focus on
materials, engineering, and application, this book is a valuable
resource for materials scientists, skin biologists, and
bioengineers with an interest in tissue engineering, regeneration,
and repair of skin.
With insolubility proving to be one of the most crippling
bottlenecks in the protein production and purification process,
this volume serves to aid researchers working in the recombinant
protein production field by describing a wide number of protocols
and examples. Insoluble Proteins: Methods and Protocols includes
chapters that describe not only the recombinant protein production
in different expression systems but also different purification and
characterization methods to finally obtain these
difficult-to-obtain proteins. Beginning with protein production
methods using both prokaryotic and eukaryotic expression systems,
the book continues with purification protocols using insoluble
proteins, the characterization of insoluble proteins, as well as a
general overview of interesting applications of insoluble proteins.
Written in the highly successful Methods in Molecular Biology
series format, chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Comprehensive and
practical, Insoluble Proteins: Methods and Protocols aims to
provide the scientific community with detailed and reliable
state-of-the-art protocols that are used in order to successfully
produce and purify recombinant proteins prone to aggregate.
Walking machines have potential advantages over traditional
vehicles, and they have already succeeded in carrying out many
tasks that wheeled or tracked robots cannot handle. Nevertheless,
their use in industry and services is currently limited in scope.
Quadrupedal Locomotion: An Introduction to the Control of
Four-legged Robots brings together some of the methods and
techniques in this emerging field that have recently been developed
in an effort to deal with the problems that currently prevent
legged robots being more widely used for real applications.
Quadrupedal Locomotion: An Introduction to the Control of
Four-legged Robots illustrates the appropriate algorithms and
methods through a discussion of simulation and experiments that
have been tested on a real machine, the SILO4 walking robot. Data
from the experiments can be found on-line at http:
//www.iai.csic.es/users/silo4/. This book is divided into two
parts: the first part, Walking Measurements and Algorithms,
introduces the historical development of quadrupeds, their
advantages/disadvantages and potential uses, and the trade-off
between quadrupeds and hexapods. The second part, Control
Techniques, concentrates on general techniques that have been
specifically applied to legged robots, including kinematic and
dynamic models, soft computing techniques to increase speed,
virtual sensors that help reduce the electronic burden of the
machine, and software simulators to study and test certain robot
properties.
As the first book to focus specifically on quadrupeds,
Quadrupedal Locomotion: An Introduction to the Control of
Four-legged Robots will be suitable for researchers, postgraduates
and senior undergraduates in the field of robotics as well as
engineers working in industry.
Set against conventional views of Peru as a place where indigenous
mobilization has been absent, this book examines the complex,
contentious politics between intercultural activists, local Andean
indigenous community members, state officials, non-governmental
organizations, and transnationally-educated indigenous
intellectuals. It examines the paradoxes and possibilities of
Quechua community protests against intercultural bilingual
education, official multicultural policies implemented by state and
non-state actors, and the training of "authentic" indigenous
leaders far from their home communities. Focusing on important
local sites of transnational connections, especially in the
highland communities of Cuzco, and on an international academic
institute for the study of intercultural bilingual education, this
book shows how contemporary indigenous politics are inextricably
and simultaneously local and global. In exploring some of the
seeming contradictions of Peruvian indigenous politics, Making
Indigenous Citizens suggests that indigenous movements and
citizenship are articulated in extraordinary but under-explored
ways in Latin America and beyond.
This volume serves to aid researchers working in the recombinant
protein production field by describing a wide number of protocols
and examples. Chapters describe recombinant protein production in
different expression systems, prokaryotic and eukaryotic expression
systems, purification protocols, characterization of insoluble
proteins and a general overview of interesting applications of
insoluble proteins. Written in the format of the highly successful
Methods in Molecular Biology series, each chapter includes an
introduction to the topic, lists necessary materials and reagents,
includes tips on troubleshooting and known pitfalls, and
step-by-step, readily reproducible protocols. Authoritative and
cutting-edge, Insoluble Proteins: Methods and Protocols, Second
Edition aims to be a useful practical guide to researches to help
further their study in this field.
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