This book addresses the complexity of mixed language classroom learning environments in which heritage learners (HL) and second language (L2) learners are concurrently exposed to language learning in the same physical space. Heritage speakers, defined widely as those exposed to the target language at home from an early age, tend to display higher oral proficiency and increased intercultural proficiency but lesser metalinguistic and grammatical awareness than L2 learners. The theoretical and pedagogical challenges of engaging both types of learners simultaneously without polarizing the classroom community dictates the need for well-defined, differentiated learning strategies; in response this book offers best practices and reproducible pedagogical initiatives and methodologies for different levels of instruction. The chapters address themes including translanguaging, linguistic identity, metalinguistic awareness and intercultural competence, with contributions from Europe, Africa and the United States.

This book addresses the complexity of mixed language classroom learning environments in which heritage learners (HL) and second language (L2) learners are concurrently exposed to language learning in the same physical space. Heritage speakers, defined widely as those exposed to the target language at home from an early age, tend to display higher oral proficiency and increased intercultural proficiency but lesser metalinguistic and grammatical awareness than L2 learners. The theoretical and pedagogical challenges of engaging both types of learners simultaneously without polarizing the classroom community dictates the need for well-defined, differentiated learning strategies; in response this book offers best practices and reproducible pedagogical initiatives and methodologies for different levels of instruction. The chapters address themes including translanguaging, linguistic identity, metalinguistic awareness and intercultural competence, with contributions from Europe, Africa and the United States.

This volume serves to aid researchers working in the recombinant protein production field by describing a wide number of protocols and examples. Chapters describe recombinant protein production in different expression systems, prokaryotic and eukaryotic expression systems, purification protocols, characterization of insoluble proteins and a general overview of interesting applications of insoluble proteins. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Insoluble Proteins: Methods and Protocols, Second Edition aims to be a useful practical guide to researches to help further their study in this field.

With insolubility proving to be one of the most crippling bottlenecks in the protein production and purification process, this volume serves to aid researchers working in the recombinant protein production field by describing a wide number of protocols and examples. Insoluble Proteins: Methods and Protocols includes chapters that describe not only the recombinant protein production in different expression systems but also different purification and characterization methods to finally obtain these difficult-to-obtain proteins. Beginning with protein production methods using both prokaryotic and eukaryotic expression systems, the book continues with purification protocols using insoluble proteins, the characterization of insoluble proteins, as well as a general overview of interesting applications of insoluble proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Insoluble Proteins: Methods and Protocols aims to provide the scientific community with detailed and reliable state-of-the-art protocols that are used in order to successfully produce and purify recombinant proteins prone to aggregate.

Walking machines have potential advantages over traditional vehicles, and they have already succeeded in carrying out many tasks that wheeled or tracked robots cannot handle. Nevertheless, their use in industry and services is currently limited in scope. Quadrupedal Locomotion: An Introduction to the Control of Four-legged Robots brings together some of the methods and techniques in this emerging field that have recently been developed in an effort to deal with the problems that currently prevent legged robots being more widely used for real applications.

Quadrupedal Locomotion: An Introduction to the Control of Four-legged Robots illustrates the appropriate algorithms and methods through a discussion of simulation and experiments that have been tested on a real machine, the SILO4 walking robot. Data from the experiments can be found on-line at http: //www.iai.csic.es/users/silo4/. This book is divided into two parts: the first part, Walking Measurements and Algorithms, introduces the historical development of quadrupeds, their advantages/disadvantages and potential uses, and the trade-off between quadrupeds and hexapods. The second part, Control Techniques, concentrates on general techniques that have been specifically applied to legged robots, including kinematic and dynamic models, soft computing techniques to increase speed, virtual sensors that help reduce the electronic burden of the machine, and software simulators to study and test certain robot properties.

As the first book to focus specifically on quadrupeds, Quadrupedal Locomotion: An Introduction to the Control of Four-legged Robots will be suitable for researchers, postgraduates and senior undergraduates inthe field of robotics as well as engineers working in industry.

This work focuses on rural community versions of Spanish Early Modern Theatre and deals with cultural heritage and the contemporary impact of Golden Age theatre on local rural communities. To this end, I examine the burgeoning of annual rural Golden Age theatre festivals that generate site-centered, non-professional productions of the plays, and revisit the conflict between tradition and innovation, between popular and high culture between authority of literary heritage and the people's right to the canon. The selection of Early Modern plays set in actual Spanish communities-Fuenteovejuna, El Alcalde de Zalamea, Numancia and Los tres blasones de Espana-renders an overview of the effect of these important works on their respective communities and focuses on the theatrical festivals as peripheral, subaltern, hybrid cultural phenomena. I take into consideration not only traditional and significant studies on these four renowned plays, but recent theories on staging, performance and popular reception and agency. The research involved crosses traditional disciplinary boundaries between literature, history, geography, and politics by centering on the appropriation and re-examination of a past that is continuously revised through contemporary performance, and which is adjusted to fit the needs and desires of the context in which it is interpreted. This diachronic approach allows for a new perspective on contemporary performances which question cultural politics, redefine tradition and transcend geo-political boundaries.

Biomaterials for Skin Repair and Regeneration examines a range of materials and technologies used for regenerating or repairing skin. With a strong focus on biomaterials and scaffolds, the book also examines the testing and evaluation pathway for human clinical trials. Beginning by introducing the fundamentals on skin tissue, the book goes on to describe contemporary technology used in skin repair as well as currently available biomaterials suitable for skin tissue repair and regeneration. Skin tissue engineering and the ideal requirements to take into account when developing skin biomaterials are discussed, followed by information on the individual materials used for skin repair and regeneration. As evaluation of biomaterials in animal models is mandatory before proceeding into human clinical trials, the book also examines the different animal models available. With a strong focus on materials, engineering, and application, this book is a valuable resource for materials scientists, skin biologists, and bioengineers with an interest in tissue engineering, regeneration, and repair of skin.

With insolubility proving to be one of the most crippling bottlenecks in the protein production and purification process, this volume serves to aid researchers working in the recombinant protein production field by describing a wide number of protocols and examples. Insoluble Proteins: Methods and Protocols includes chapters that describe not only the recombinant protein production in different expression systems but also different purification and characterization methods to finally obtain these difficult-to-obtain proteins. Beginning with protein production methods using both prokaryotic and eukaryotic expression systems, the book continues with purification protocols using insoluble proteins, the characterization of insoluble proteins, as well as a general overview of interesting applications of insoluble proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Insoluble Proteins: Methods and Protocols aims to provide the scientific community with detailed and reliable state-of-the-art protocols that are used in order to successfully produce and purify recombinant proteins prone to aggregate.

Walking machines have potential advantages over traditional vehicles, and they have already succeeded in carrying out many tasks that wheeled or tracked robots cannot handle. Nevertheless, their use in industry and services is currently limited in scope. Quadrupedal Locomotion: An Introduction to the Control of Four-legged Robots brings together some of the methods and techniques in this emerging field that have recently been developed in an effort to deal with the problems that currently prevent legged robots being more widely used for real applications.

Quadrupedal Locomotion: An Introduction to the Control of Four-legged Robots illustrates the appropriate algorithms and methods through a discussion of simulation and experiments that have been tested on a real machine, the SILO4 walking robot. Data from the experiments can be found on-line at http: //www.iai.csic.es/users/silo4/. This book is divided into two parts: the first part, Walking Measurements and Algorithms, introduces the historical development of quadrupeds, their advantages/disadvantages and potential uses, and the trade-off between quadrupeds and hexapods. The second part, Control Techniques, concentrates on general techniques that have been specifically applied to legged robots, including kinematic and dynamic models, soft computing techniques to increase speed, virtual sensors that help reduce the electronic burden of the machine, and software simulators to study and test certain robot properties.

As the first book to focus specifically on quadrupeds, Quadrupedal Locomotion: An Introduction to the Control of Four-legged Robots will be suitable for researchers, postgraduates and senior undergraduates in the field of robotics as well as engineers working in industry.

Set against conventional views of Peru as a place where indigenous mobilization has been absent, this book examines the complex, contentious politics between intercultural activists, local Andean indigenous community members, state officials, non-governmental organizations, and transnationally-educated indigenous intellectuals. It examines the paradoxes and possibilities of Quechua community protests against intercultural bilingual education, official multicultural policies implemented by state and non-state actors, and the training of "authentic" indigenous leaders far from their home communities. Focusing on important local sites of transnational connections, especially in the highland communities of Cuzco, and on an international academic institute for the study of intercultural bilingual education, this book shows how contemporary indigenous politics are inextricably and simultaneously local and global. In exploring some of the seeming contradictions of Peruvian indigenous politics, Making Indigenous Citizens suggests that indigenous movements and citizenship are articulated in extraordinary but under-explored ways in Latin America and beyond.

This volume serves to aid researchers working in the recombinant protein production field by describing a wide number of protocols and examples. Chapters describe recombinant protein production in different expression systems, prokaryotic and eukaryotic expression systems, purification protocols, characterization of insoluble proteins and a general overview of interesting applications of insoluble proteins. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Insoluble Proteins: Methods and Protocols, Second Edition aims to be a useful practical guide to researches to help further their study in this field.