Molecular Toxicology Protocols, Second Edition aims to bring together a series of articles describing validated methods to elucidate specific molecular aspects of toxicology, the emphasis being on the application of molecular methods to genetic toxicology. The volume is divided into ten parts, roughly corresponding to the spectrum of biomarkers intermediate between exposure and disease outcomes as proposed in molecular epidemiology models. Subjects of these new chapters range from preparation of fluid specimens for analysis of cellular inflammatory responses to genotoxic insults to sensitive methods for proteomic analysis and aberrant DNA methylation patterns. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Molecular Toxicology Protocols, Second Edition addresses not only the needs of molecular biologists and toxicologists, but also those of individuals interested in applying molecular methods to clinical applications, such as geneticists, pathologists, biochemists, and epidemiologists.

This essential information captures the state of the composites industry to assist engineering/technical professionals in charting a course for achieving economic success. The material characteristics of composites, their applications, and complex composites manufacturing processes depend on many factors. These are all fully considered and presented to meet the challenges that face this marketplace. The expert panel of writers from various industry segments (i.e., commercial/military aerospace, wind energy, automotive, and bicycle industries) address fundamental topics and explore the affordability of composites from raw material to end-of life-disposal costs with skilled perspectives that include: Material characteristics and economics of composite structure Complex manufacturing and specifics of assembly methods Applications for composites Product and human health Safety and environmental impacts The authors provide strong basic economics concepts that are directly applied to the composites industry. The content conveys both the reality of the industry, as well as the trends and constantly emerging challenges that impact the cost of composites and are necessary for return on investment, as well as enabling the full potential of composites.

The framework sketched in this new book explains the relationship between state and capital in Italy as well as some of the major directions in macroeconomic theory. These fields encompass both Italy's entry to EMU in 1999 and the impact of Silvio Berlusconi on Italian politics and economics.

This book advocates a more thoughtful approach to urban water management. The approach involves reducing water consumption, harvesting rainwater, recycling rainwater and adopting Sustainable Drainage Systems (SuDS) where surface water is not sent straight to drains but is intercepted by features like green roofs, rain gardens, swales and ponds.Cities in particular need to change the existing linear model of water consumption and use to a more circular one in order to survive. The Water Sensitive City brings together the various specialised technical discussions that have been continuing for some time into a volume that is more accessible to designers (engineers and architects), urban planners and managers, and policymakers.

Molecular Toxicology Protocols, Second Edition aims to bring together a series of articles describing validated methods to elucidate specific molecular aspects of toxicology, the emphasis being on the application of molecular methods to genetic toxicology. The volume is divided into ten parts, roughly corresponding to the spectrum of biomarkers intermediate between exposure and disease outcomes as proposed in molecular epidemiology models. Subjects of these new chapters range from preparation of fluid specimens for analysis of cellular inflammatory responses to genotoxic insults to sensitive methods for proteomic analysis and aberrant DNA methylation patterns. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Molecular Toxicology Protocols, Second Edition addresses not only the needs of molecular biologists and toxicologists, but also those of individuals interested in applying molecular methods to clinical applications, such as geneticists, pathologists, biochemists, and epidemiologists.

Over a mere 5 years, neonatal cranial sonography has evolved from an obscure and largely experimental imaging possibility to the modality of preference in the examination of the young brain. The almost immediate acceptance of the ultrasound examination of the neonatal brain was based on a number of coinci dent factors, the most important of which was the emergence of a burgeoning population of premature neonates who were, for the first time, surviving be yond infancy. These delicate patients were beginning to withstand the rigors of extrauterine life when not fully prepared for it; pulmonary, cardiac, and infec tious diseases no longer claimed most of them. With survival, a new specter reared its head: Would the eventual mental and neurologic status of these same children be worth the expense and time needed to bring them through their first months? This issue became increasingly pressing as evidence mounted through the 1970s that very premature neonates were at a high risk for intracranial hemor rhage and posthemorrhagic complications. An imaging modality that could evaluate the premature brain was sorely needed. The CT scanner with its proven ability to diagnose intracranial hemorrhage was of little value in this regard. So too were static gray-scale or waterpath ultrasound units. These modalities all had the same limitation, lack of portability. As neonatal intensive care units proliferated, so did the technology that would soon allow cribside neonatal neuroimaging, the real-time sector scanner."

"Duplex Sonography" is the first comprehensive text written about this modality. The book offers the reader detailed information about all major uses of duplex and is introduced by a brief chapter on the physical principles of doppler ultrasound as it relates to duplex scanning. "Duplex " "Sonography" is intended to provide relevant information on all aspects of the technique, ranging from the basics of performing the examination to the features of sometimes complex pathological states. The book is intended for anyone interested in non-invasive vascular diagnosis including radiologists, vascular surgeons and ultrasound/peripheral vascular technologists. Other groups may find individual chapters appealing: carotid/cardiac sonography for cardiologists, fetal sonography for obstetricians or carotid sonography for neurologists. Each chapter is not only a guide to duplex evaluation, but also provides valuable information about vascular dynamics of the organ system under discussion. Physicians or technologists reading this book should come away with a well-rounded background in state-of-the-art duplex sonography and will undoubtedly discover new possibilities for using this non-invasive vascular technique.

A collection of cutting-edge techniques for analyzing genotoxic exposure and detecting the resulting biological effects-including endogenous metabolites-up to and including the development of cancer. The authors emphasize analytical methods that can be specifically applied to human populations and patients. Among the applications detailed are the analysis of interactions between such cellular macromolecules as DNA and proteins and chemical and physical agents, the assessment of medically relevant toxicity, and the characterization of genetic alterations induced in transgenic animals by in vivo systems. There are also methods for the analysis of genotoxic exposure during gene expression, of cytotoxicity caused by the induction of apoptosis, of genetic alterations in reporter genes and oncogenes, early (premalignant) detection of altered oncogenes, and of individual variation in biotransformation and DNA repair capacity.

The springboard for this sixth volume in the Industry and Health Care series was a conference sponsored by the Center for Industry and Health Care of Boston University on June 9 and 10, 1978. That conference had a gradual genesis. Over a year ago we spent some time with Kevin Stokeld of Deere and Company and heard his views on self-insurance and self-administration as one device for a corporation to achieve better management control of its health benefit. More recent discussions with representatives of American Telephone and Telegraph Company and other corporations made it increasingly clear to us that management's need for data to monitor the use of employee health benefits was emerging as a critical policy issue. Subsequent meetings with executives at John Hancock Mutual Life Insurance Company in Boston and Mobil Oil Corporation in New York, among others, convinced us that simple answers would be elusive or inadequate and that there was a need for an objective and careful look at the evolving relationships between employee health benefits, claims administration, health services utilization, and corpo rate health care cost containment programs. Since self-funding and particularly self-administration represent a fun damental change in the traditional insurance relationship, the conference was convened to explore the advantages and disadvantages of self-insurance for employee health benefits, with some attention to claims production but with special emphasis on the originating question of data for effective management of an employee health benefit."

The need to find new approaches to the development of cities is becoming increasingly urgent in this age of continuing population growth, demographic transition, climate change, fossil fuel peak and biodiversity losses. Restoring ecosystem services and promoting biodiversity is essential to sustainable development - even in the built environment.

"Ecosystem Services come to Town: greening cities by working with nature "demonstrates how to make urban environments greener. It starts by explaining how, by mimicking nature and deliberately creating habitats to provide ecosystem services, cities can become more efficient and more pleasant to live in. The history of cities and city planning is covered with the impacts of industrial urban development described, as well as the contemporary concerns of biodiversity loss, peak oil and climate change.

The later sections offer solutions to the challenges of sustainable urban development by describing and explaining a whole range of approaches and interventions, beginning at the regional scale with strategic green infrastructure, looking at districts and precincts, with trees, parks and rain gardens and ending with single buildings, including with green roofs and living walls.

Technical enough to be valuable to practitioners but still readable and inspirational, this guide demonstrates to town planners, urban designers, architects, engineers, landscape architects how to make cities more liveable.

Ultrasound is used frequently as the modality of choice for vascular imaging.? It's unique ability to demonstrate blood flow make it particularly effective.? Reviewed in this issue are the US applications in the imaging of the heart, kidneys, and liver.? Also covered are imaging of US for imaging of the carotid artery and in deep vein thrombosis.? Lastly an article highlighting ways to optimize Doppler US is included.