This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.

The book presents a unified treatment of integer programming and network models with topics ranging from exact and heuristic algorithms to network flows, traveling salesman tours, and traffic assignment problems. While the emphasis of the book is on models and applications, the most important methods and algorithms are described in detail and illustrated by numerical examples. The formulations and the discussion of a large variety of models provides insight into their structures that allows the user to better evaluate the solutions to the problems.

The purpose of this book is to provide readers with an introduction to the very active field of integer programming and network models. The idea is to cover the main parts of the field without being too detailed or too technical. As a matter of fact, we found it somewhat surprising that most--especially newer---books are strongly algorithmically oriented. In contrast, the main emphasis of this book is on models rather than methods. This focus expresses our view that methods are tools to solve actual problems and not ends in themselves. As such, graduate (and with some omissions, undergraduate) students may find this book helpful in their studies as will practitioners who would like to get acquainted with a field or use this text as a refresher. This premise has resulted in a coverage that omits material that is standard fare in other books, whereas it covers topics that are only infrequently found elsewhere. There are some, yet relatively few, prerequisites for the reader. Most material that is required for the understanding of more than one chapter is presented in one of the four chapters of the introductory part, which reviews the main results in linear programming, the analysis of algorithms, graphs and networks, and dynamic programming, respectively. Readers who are familiar with the issues involved can safely skip that part. The three main parts of the book rely on intuitive reasoning and examples, whenever practical, instead of theorems and proofs.