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Books > Science & Mathematics > Biology, life sciences > Botany & plant sciences > Plant reproduction & propagation
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Low-Cost Methods for Molecular Characterization of Mutant Plants - Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols (Paperback, Softcover reprint of the original 1st ed. 2015)
Loot Price: R1,203
Discovery Miles 12 030
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Low-Cost Methods for Molecular Characterization of Mutant Plants - Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols (Paperback, Softcover reprint of the original 1st ed. 2015)
Supplier out of stock. If you add this item to your wish list we will let you know when it becomes available.
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This book offers low-cost and rapid molecular assays for the
characterization of mutant plant germplasm. Detailed protocols are
provided for the desiccation of plant tissues; the extraction of
high-quality DNA for downstream applications; the extraction of
single-strand-specific nucleases for single nucleotide
polymorphism; and small insertion/deletion discovery using standard
agarose gel electrophoresis. The methods described can be applied
in any laboratory equipped for basic molecular biology and do away
with the need for expensive freezers and toxic organic compounds.
With the appropriate validation of sample quality and longevity,
they can provide sufficient DNA for a variety of molecular
applications, such as marker studies and TILLING, at approximately
one tenth of the cost per sample when compared to commercial kits.
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