This is the first book devoted to radiowave propagation over land and sea. Researchers and engineers involved in propagation studies and applications in communications, broadcasting, radar and remote sensing will find this volume invaluable.

Ever since the start of Chinese linguistic studies, the description of the Chinese particle LE has remained elusive. The classification has evolved from a listing of sentences and the discussion of contrastive pairs to a more context and discourse-oriented analysis. The development in recent years of inferential models and situation semantics has opened the way for a renewed study of the use of the Chinese particle LE. This book discusses the Chinese data from a 'mental space' perspective and finally reveals the role so-called Chinese 'sentence LE' plays in the construction and maintenance of discourse.

Ever since the start of Chinese linguistic studies, the description of the Chinese particle LE has remained elusive. The classification has evolved from a listing of sentences and the discussion of contrastive pairs to a more context and discourse-oriented analysis. The development in recent years of inferential models and situation semantics has opened the way for a renewed study of the use of the Chinese particle LE. The present book discusses the Chinese data from a 'mental space' perspective and brings out the role so-called Chinese 'sentence LE' plays in the construction and maintenance of discourse.

Essential principles and practice of assay development

The first comprehensive, integrated treatment of the subject, Assay Development: Fundamentals and Practices covers the essentials and techniques involved in carrying out an assay project in either a biotechnology/drug discovery setting or a platform setting.

Rather than attempting comprehensive coverage of all assay development technologies, the book introduces the most widely used assay development technologies and illustrates the art of assay development through a few commonly encountered biological targets in assay development (e.g., proteases, kinases, ion channels, and G protein-coupled receptors). Just enough biological background for these biological targets is provided so that the reader can follow the logics of assay development. Chapters discuss:

The basics of assay development, including foundational concepts and applications

Commonly used instrumental methods for both biochemical assays and cell-based assays

Assay strategies for protein binding and enzymatic activity

Cell-based assays

High-throughput screening

An in-depth study of the now popular Caliper's off-chip kinase assay provides an instructive, real-world example of the assay development process.