Essential principles and practice of assay development
The first comprehensive, integrated treatment of the subject,
Assay Development: Fundamentals and Practices covers the essentials
and techniques involved in carrying out an assay project in either
a biotechnology/drug discovery setting or a platform setting.
Rather than attempting comprehensive coverage of all assay
development technologies, the book introduces the most widely used
assay development technologies and illustrates the art of assay
development through a few commonly encountered biological targets
in assay development (e.g., proteases, kinases, ion channels, and G
protein-coupled receptors). Just enough biological background for
these biological targets is provided so that the reader can follow
the logics of assay development. Chapters discuss:
The basics of assay development, including foundational concepts
and applications
Commonly used instrumental methods for both biochemical assays
and cell-based assays
Assay strategies for protein binding and enzymatic activity
Cell-based assays
High-throughput screening
An in-depth study of the now popular Caliper's off-chip kinase
assay provides an instructive, real-world example of the assay
development process.
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