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Mitogen-activated protein kinases (MAPKs) are versatile
phosphorylating enzymes which regulate multiple proteins involved
in gene expression, cell architecture, plant development and
reaction to diverse abiotic and biotic factors. The main aim of
Plant MAP Kinases: Methods and Protocols is to provide established
and new MAPK protocols adapted to the challenges posed by working
with plants. The book contains 19 chapters which encompass a wide
array of methods progressively scaling from the single gene,
protein or cell level to large-scale arrays of proteomic,
phosphoproteomic and interactomic data in order to uncover
previously unidentified plant MAPK signaling pathways and to tackle
with the challenging task of substrate identification. Techniques
for MAPK sequence analysis and subcellular localization helping to
identify their substrates and subcellular compartmentalization are
also provided. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols
and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant MAP Kinases: Methods and
Protocols represents a collection of useful plant MAPK protocols
written by experts in the field for researchers and students.
Mitogen-activated protein kinases (MAPKs) are versatile
phosphorylating enzymes which regulate multiple proteins involved
in gene expression, cell architecture, plant development and
reaction to diverse abiotic and biotic factors. The main aim of
Plant MAP Kinases: Methods and Protocols is to provide established
and new MAPK protocols adapted to the challenges posed by working
with plants. The book contains 19 chapters which encompass a wide
array of methods progressively scaling from the single gene,
protein or cell level to large-scale arrays of proteomic,
phosphoproteomic and interactomic data in order to uncover
previously unidentified plant MAPK signaling pathways and to tackle
with the challenging task of substrate identification. Techniques
for MAPK sequence analysis and subcellular localization helping to
identify their substrates and subcellular compartmentalization are
also provided.Written in the highly successful Methods in Molecular
Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and key
tips on troubleshooting and avoiding known pitfalls. Authoritative
and practical, Plant MAP Kinases: Methods and Protocols represents
a collection of useful plant MAPK protocols written by experts in
the field for researchers and students.
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