Analysis of the neural tissue presents unique and peculiar technical problems encountered in everyday bench work. Numerous books dealing with cellular and molecular protocols for general use in cell biology are available, but few are specifically devoted exclusively to neurobiology. Moreover, the "cross-talk" between researchers with different backgrounds, i.e. histologists, cell and molecular biologists and physiologists, is still quite difficult, and very often one remains somehow "confined" to his or her own specific field of expertise never daring to explore "mysterious" lands unless having the support of a big laboratory beyond. The general idea beyond this project was to put together the contributions from a number of well-known neuroscientists to produce a book that offers a survey of the most updated techniques for the study of nerve cells. After a long time spent doing research in the neuroscience field, and having acquired a good technical background in certain specific fields of neurobiology we have realized how difficult is to be able to step into a different technology. This book endeavors to assist in that goal.