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This volume covers the latest technological advances in the
characterization of the biosynthesis and functions of Rab GTPases
and their regulation by guanine nucleotide exchange factors (GEFs)
and GTPase-activating proteins (GAPs). The book consists of 28
chapters and starts with an overview of the Rab GTPase family. The
next few chapters describe systematic approaches to the
identification and classification of Rabs and Rab GAPs, as well as
the detection of Rab isoprenylation and membrane distribution. The
last few chapters examine the biochemical and functional properties
of individual Rabs in the order of exocytic, recycling and
endocytic Rabs. Written in the highly successful Methods of
Molecular Biology series format, chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols
and key tips on troubleshooting and avoiding known pitfalls.
Instructive and practical, Rab GTPases: Methods and Protocols
approaches each topic with great detail and is a valuable resource
for researchers and students interested in the field of Rab
GTPases.
This second edition volume expands on the previous edition with a
discussion of new research and discoveries in the Rab field.
Chapters in this book cover topics such as new information on Rab
regulation and localization; interaction; function; and diseases.
Written in the highly successful Methods in Molecular Biology
series format, chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Cutting-edge and
comprehensive, Rab GTPases: Methods and Protocols, Second Edition
is a valuable resource for scientists working in the fields of Rab
and other small GTPases, and beyond.
This second edition volume expands on the previous edition with a
discussion of new research and discoveries in the Rab field.
Chapters in this book cover topics such as new information on Rab
regulation and localization; interaction; function; and diseases.
Written in the highly successful Methods in Molecular Biology
series format, chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Cutting-edge and
comprehensive, Rab GTPases: Methods and Protocols, Second Edition
is a valuable resource for scientists working in the fields of Rab
and other small GTPases, and beyond.
This volume covers the latest technological advances in the
characterization of the biosynthesis and functions of Rab GTPases
and their regulation by guanine nucleotide exchange factors (GEFs)
and GTPase-activating proteins (GAPs). The book consists of 28
chapters and starts with an overview of the Rab GTPase family. The
next few chapters describe systematic approaches to the
identification and classification of Rabs and Rab GAPs, as well as
the detection of Rab isoprenylation and membrane distribution. The
last few chapters examine the biochemical and functional properties
of individual Rabs in the order of exocytic, recycling and
endocytic Rabs. Written in the highly successful Methods of
Molecular Biology series format, chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols
and key tips on troubleshooting and avoiding known pitfalls.
Instructive and practical, Rab GTPases: Methods and Protocols
approaches each topic with great detail and is a valuable resource
for researchers and students interested in the field of Rab
GTPases.
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