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This volume in the well-established "Methods in Enzymology" series
features methods for the study of lipids using mass spectrometry
techniques. Articles in this volume cover topics such as
Qualitative Analysis and Quantitative Assessment of Changes in
Neutral Glycerol Lipid Molecular Species within Cells;
Glycerophospholipid identification and quantitation by electrospray
ionization mass spectrometry; Detection and Quantitation of
Eicosanoids via High Performance Liquid Chromatography/Electrospray
Ionization Mass Spectrometry; Structure-specific, quantitative
methods for "lipidomic" analysis of sphingolipids by tandem mass
spectrometry; Analysis of Ubiquinones, Dolichols and Dolichol
Diphosphate-Oligosaccharides by Liquid Chromatography Electrospray
Ionization Mass Spectrometry; Extraction and Analysis of Sterols in
Biological Matrices by High-Performance Liquid Chromatography
Electrospray Ionization Mass Spectrometry; The Lipid Maps
Initiative in Lipidomics; Basic analytical systems for lipidomics
by mass spectrometry in Japan; The European Lipidomics Initiative
Enabling technologies; Lipidomic analysis of Signaling Pathways;
Bioinformatics for Lipidomics; Mediator Lipidomics: Search
Algorithms for Eicosanoids, Resolvins and Protectins; A guide to
biochemical systems modeling of sphingolipids for the biochemist;
and Quantitation and Standardization of Lipid Internal Standards
for Mass Spectroscopy.
This volume in the well-established Methods in Enzymology series
features methods for the study of lipids using mass spectrometry
techniques. Articles in this volume cover topics such as
Phospholipase A1 assays using a radio-labeled substrate and mass
spectrometry; Real-time Cell Assays of Phospholipases A2 Using
Fluorogenic Phospholipids; Analysis and Pharmacological Targeting
of Phospholipase C a interactions with G proteins; Biochemical
Analysis of Phospholipase D.; Measurement of
Autotaxin/Lysophospholipase D Activity; Platelet-Activating Factor;
Quantitative measurement of PtdIns(3,4,5)P3; Measuring
Phosphorylated Akt And Other Phosphoinositide 3-Kinase-Regulated
Phosphoproteins In Primary Lymphocytes; Regulation of
Phosphatidylinositol 4-Phosphate 5-Kinase activity by partner
proteins; Biochemical Analysis of Inositol Phosphate Kinases;
Analysis of the phosphoinositides and their aqueous metabolites;
Combination of C17-sphingoid base homologues and mass spectrometry
analysis as a new approach to study sphingolipid metabolism;
Measurement of mammalian sphingosine-1-phosphate phosphohydrolase
activity in vitro and in vivo; A rapid and sensitive method to
measure secretion of sphingosine-1-phosphate; Ceramide Kinase and
Ceramide-1-Phosphate; Measurement of Mammalian Diacylglycerol
Kinase Activity in vitro and in Cells; Lipid Phosphate Phosphatases
from Saccharomyces cerevisiae.
This volume in the well-established Methods in Enzymology series
features methods for the study of lipids using mass spectrometry
techniques. Articles in this volume cover topics such as Liquid
chromatography mass spectrometry for quantifying plasma
lysophospholipids: potential biomarkers for cancer diagnosis;
Measurement of eicosanoids in cancer tissues; Noninvasive
Assessment of the Role of Cyclooxygenases in Cardiovascular
Health
A Detailed HPLC/MS/MS Method; Lipidomics in Diabetes and the
Metabolic Syndrome; LC-MS-MS Analysis of Neutral Eicosanoids;
Quantification Of F2-Isoprostanes In Biological Fluids And Tissues
As A Measure Of Oxidant Stress; Measurement of Products of
Docosahexaenoic Acid Peroxidation, Neuroprostanes, and Neurofurans;
Enantiomeric separation of hydroxy and hydroperoxy eicosanoids by
chiral column chromatography; Targeted Chiral Lipidomics Analysis
by Liquid Chromatography Electron Capture Atmospheric Pressure
Chemical Ionization Mass Spectrometry (LC-ECAPCI/MS); Shotgun
Lipidomics by Tandem Mass Spectrometry under Data-Dependent
Acquisition Control; Identification of Intact Lipid Peroxides by
Ag+ Coordination Ionspray Mass Spectrometry (CIS-MS);
Quantification of Cardiolipin by Liquid Chromatography Electrospray
Ionization Mass Spectrometry.
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