13C- and H-MRS of Cultured Neurones and Glia; U. Sonnewald, et al. Measurement of Intracellular Cations; H. Bachelard, R. Badar-Goffer. In Vivo Nitrogen MRS Studies of Rat Brain Metabolism; K. Kanamori, B.D. Ross. Traumatic Brain Injury; R. Vink, T.K. McIntosh. Animal Models of Stroke; T.L.C. Luvisotto, G.R. Sutherland. In Vivo Magnetic Resonance Imaging and Spectroscopy: Application to Brain Tumors; B.D. Ross, et al. Diffusion-Weighted Magnetic Resonance Imaging; M. King, et al. High Speed Echo Planar Imaging and Its Application to Stroke; P. Gowland, P. Mansfield. Brain Activation Studies Using Magnetic Resonance Imaging; S. Williams, et al. MRI and Proton MRS in the Evaluation of Multiple Sclerosis; D.L. Arnold, et al. Phosphorous and Proton Magnetic Resonance Spectroscopy of the Brain of the Newborn Human Infant; E.B. Cady. Localized 1H-MRS in Brain Disorders; J. Frahm, F. Hanefeld. Glossary. Index.

Neurobiology has undergone a revolution with many advances dependent on new techniques and approaches. This fully updated second edition reflects the recent growth areas in this subject. Its major themes are the fractionation and study of cellular and subcellular components of the nervous system, chemical neuroanatomy, and the measurement of neuronal metabolism in vivo and in vitro. Topics include the neuronal cytoskeleton; isolation of synaptosomes and their subcellular components; aspects of mitochondrial defects in neurones; and synaptic transport processes exemplified by the GABA transporter. This is an invaluable practical laboratory manual for all researchers in neurobiology; it contains step-by-step protocols and a troubleshooting guide, as well as examples of experimental data and their interpretation.

The brain is the most complex and highly of semi-permeable excitable membranes which specialised of all mammalian organs. Under can be caused, rapidly and transiently, to under standing the complexity of its function remains go changes in permeability to small chemical man's greatest challenge. The functional unit is molecules and to cations. The highly specialised the neurone, or excitable nerve cell, making ana nature of the constituent cells, with their unique tomical and chemical connections with other function and specificity, is c10sely related to units in the system. Many of the essential bio the structure of the whole tissue. The underlying chemical connections of the nerve cell are de chemical processes cannot be discussed or seen pendent upon special morphological features: in perspective without constant awareness of synaptic contact is mediated by chemical mol related aspects of physiology and morphology. ecules, 'neuro-transmitters' which ensure the The hrain is structurally extraordinarily com continued propagation of electrical impulses plex in its distinct anatomical regions, each of through sequential units of the system. Also which is heterogeneous in the types and struc c10sely related to the morphology of the ner tures of the constituent cells. vous system is the chemical energy expended in One aspect of the biochemical function of maintaining distribution gradients of cations the brain can be seen in its efficient production across cellular membranes. Chemical neuro of the energy required to support the unique pro transmission results in an alteration in cation cesses referred to above.