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Nuclear Functions in Plant Transcription, Signaling and Development (Hardcover, 2015 ed.): Olga Pontes, Hailing Jin Nuclear Functions in Plant Transcription, Signaling and Development (Hardcover, 2015 ed.)
Olga Pontes, Hailing Jin
R4,347 R3,490 Discovery Miles 34 900 Save R857 (20%) Ships in 12 - 19 working days

The genome is more than a linear code as depicted by its DNA sequences as several interacting factors play a crucial role in shaping its organization and function. The complete sequences of a number of plant genomes and the recent advances of high-throughput technologies has fueled research efforts in the field of Plant Nuclear Biology unveiling numerous insights about the mechanisms underlying genome regulation. Genomic information is being integrated into molecular- and cellular-level mechanisms of the plant processes. A host of nuclear processes underlie key developmental processes as well as biotic and abiotic interactions. Non-coding RNAs have been increasingly recognized as players in gene expression and genome defense and integrity. However, in vivo, genomes exist as elaborate physical structures, and their functional properties are strongly determined by their cellular organization. Various types of subcellular structure have been identified in the nucleus, which are associated with transcription factors, RNA processing proteins and epigenetic regulators. Interestingly, these nuclear bodies display different behaviors in response to the environment. This book compiles a series of landmark discussions of the recent advances in plant nuclear biology research focusing in the functional relevance of the arrangement of genomes and nuclear processes that impact plant physiology and development.

RNA Abundance Analysis - Methods and Protocols (Hardcover, 2012 ed.): Hailing Jin, Walter Gassmann RNA Abundance Analysis - Methods and Protocols (Hardcover, 2012 ed.)
Hailing Jin, Walter Gassmann
R2,922 Discovery Miles 29 220 Ships in 10 - 15 working days

RNA abundance analysis is one of the most important approaches for gene expression studies in the field of molecular biology. In "RNA Abundance Analysis: Methods and Protocols," expert researchers cover a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches. This volume includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, sulfur-containing RNAs, viral and satellite RNAs, RNA isoforms, and alternatively spliced RNA variants from various organisms, as well as key discussions of computational data processing for genome-wide datasets. Written for the highly successful "Methods in Molecular Biology" series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Essential and easy to use, "RNA Abundance Analysis: Methods and Protocols" provides a comprehensive set of techniques and methods on isolating and analyzing mRNAs, small RNAs, and modified RNAs, which can assist you in your gene expression studies."

RNA Abundance Analysis - Methods and Protocols (Hardcover, 2nd ed. 2021): Hailing Jin, Isgouhi Kaloshian RNA Abundance Analysis - Methods and Protocols (Hardcover, 2nd ed. 2021)
Hailing Jin, Isgouhi Kaloshian
R4,391 Discovery Miles 43 910 Ships in 10 - 15 working days

This updated book covers a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches. It includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, organelle RNAs, endosymbiont RNAs, and alternatively spliced RNA variants from various organisms. RNA editing and the computational data processing for genome-wide datasets is also discussed. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, RNA Abundance Analysis: Methods and Protocols, Second Edition provides a comprehensive set of techniques and methods on isolating and analyzing mRNAs, small RNAs, and other RNA variants, in order to assist researchers in their gene expression studies.

Nuclear Functions in Plant Transcription, Signaling and Development (Paperback, Softcover reprint of the original 1st ed.... Nuclear Functions in Plant Transcription, Signaling and Development (Paperback, Softcover reprint of the original 1st ed. 2015)
Olga Pontes, Hailing Jin
R3,373 Discovery Miles 33 730 Ships in 10 - 15 working days

The genome is more than a linear code as depicted by its DNA sequences as several interacting factors play a crucial role in shaping its organization and function. The complete sequences of a number of plant genomes and the recent advances of high-throughput technologies has fueled research efforts in the field of Plant Nuclear Biology unveiling numerous insights about the mechanisms underlying genome regulation. Genomic information is being integrated into molecular- and cellular-level mechanisms of the plant processes. A host of nuclear processes underlie key developmental processes as well as biotic and abiotic interactions. Non-coding RNAs have been increasingly recognized as players in gene expression and genome defense and integrity. However, in vivo, genomes exist as elaborate physical structures, and their functional properties are strongly determined by their cellular organization. Various types of subcellular structure have been identified in the nucleus, which are associated with transcription factors, RNA processing proteins and epigenetic regulators. Interestingly, these nuclear bodies display different behaviors in response to the environment. This book compiles a series of landmark discussions of the recent advances in plant nuclear biology research focusing in the functional relevance of the arrangement of genomes and nuclear processes that impact plant physiology and development.

RNA Abundance Analysis - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed. 2012): Hailing Jin, Walter... RNA Abundance Analysis - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed. 2012)
Hailing Jin, Walter Gassmann
R3,591 Discovery Miles 35 910 Ships in 10 - 15 working days

RNA abundance analysis is one of the most important approaches for gene expression studies in the field of molecular biology. In RNA Abundance Analysis: Methods and Protocols, expert researchers cover a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches. This volume includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, sulfur-containing RNAs, viral and satellite RNAs, RNA isoforms, and alternatively spliced RNA variants from various organisms, as well as key discussions of computational data processing for genome-wide datasets. Written for the highly successful Methods in Molecular Biology (TM) series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Essential and easy to use, RNA Abundance Analysis: Methods and Protocols provides a comprehensive set of techniques and methods on isolating and analyzing mRNAs, small RNAs, and modified RNAs, which can assist you in your gene expression studies.

RNA Abundance Analysis - Methods and Protocols (Paperback, 2nd ed. 2021): Hailing Jin, Isgouhi Kaloshian RNA Abundance Analysis - Methods and Protocols (Paperback, 2nd ed. 2021)
Hailing Jin, Isgouhi Kaloshian
R3,124 Discovery Miles 31 240 Ships in 10 - 15 working days

This updated book covers a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches. It includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, organelle RNAs, endosymbiont RNAs, and alternatively spliced RNA variants from various organisms. RNA editing and the computational data processing for genome-wide datasets is also discussed. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, RNA Abundance Analysis: Methods and Protocols, Second Edition provides a comprehensive set of techniques and methods on isolating and analyzing mRNAs, small RNAs, and other RNA variants, in order to assist researchers in their gene expression studies.

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