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In this laboratory "cook-book," the authors provide a concise guide
to PCR-based techniques to quantify nucleic acids in biological and
clinical samples using exclusively nonradioactive detection
methods, e.g. HPLC, biotin and digoxigenin based protocols. Each
method presentation also includes sections on theory, reagents,
standards, applicability, limitations, and trouble shooting. In
addition to the protocols, the authors also provide the necessary
information on: general aspects of nucleic acid quantitation;
design of PCR standards; mRNA purification; cDNA synthesis;
solution hybridization; DNA sequencing. This laboratory guide
enables professionals as well as beginners to adopt easily
quantitative PCR protocols into their own clinical or biomedical
research.
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