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Analytical ultracentrifugation (AUC) can supply rich information on
the mass, shape, size distribution, solvation, and composition of
macromolecules and nanoscopic particles. It also provides a
detailed view of their reversible single- or multi-component
interactions over a wide range of affinities. Yet this powerful
technique has been hard to master in mainstream molecular sciences
due to a lack of comprehensive books on the subject. Filling this
gap in the literature of biophysical methodology, Basic Principles
of Analytical Ultracentrifugation explains the fundamentals in the
theory and practice of AUC. The book provides you with up-to-date
experimental information to confidently practice AUC. You will
understand the basic concepts, full potential, and possible
pitfalls of AUC as well as appreciate the current relevance of past
work in the field. The book first introduces the basic principles
and technical setup of an AUC experiment and briefly describes the
optical systems used for detection. It then explores the
ultracentrifugation experiment from a macromolecular standpoint,
offering a detailed physical picture of the sedimentation process
and relevant macromolecular parameters. The authors present
important practical aspects for conducting an experiment, including
sample preparation, data acquisition and data structure, and the
execution of the centrifugal experiment. They also cover instrument
calibration and quality control experiments.
Analytical ultracentrifugation is one of the most powerful solution
techniques for the study of macromolecular interactions, to define
the number and stoichiometry of complexes formed, and to measure
affinities ranging from very strong to very weak and repulsive.
Building on the data analysis tools described in the volume
Sedimentation Velocity Analytical Ultracentrifugation: Discrete
Species and Size-Distributions of Macromolecules and Particles, and
the experimental and instrumental aspects in the first volume Basic
Principles of Analytical Ultracentrifugation, the present volume
Sedimentation Velocity Analytical Ultracentrifugation: Interacting
Systems is devoted to the theory and practical data analysis of
dynamically coupled sedimentation processes. This volume is
designed to fill a gap in biophysical methodology to provide a
framework that builds on the fundamentals of the highly developed
traditional methods of analytical ultracentrifugation, updated with
current methodology and from a viewpoint of modern applications. It
will be an invaluable resource for researchers and graduate
students interested in the application of analytical
ultracentrifugation in the study of interacting systems, such as
biological macromolecules, multi-protein complexes, polymers, or
nanoparticles.
Analytical ultracentrifugation (AUC) can supply rich information on
the mass, shape, size distribution, solvation, and composition of
macromolecules and nanoscopic particles. It also provides a
detailed view of their reversible single- or multi-component
interactions over a wide range of affinities. Yet this powerful
technique has been hard to master in mainstream molecular sciences
due to a lack of comprehensive books on the subject. Filling this
gap in the literature of biophysical methodology, Basic Principles
of Analytical Ultracentrifugation explains the fundamentals in the
theory and practice of AUC. The book provides you with up-to-date
experimental information to confidently practice AUC. You will
understand the basic concepts, full potential, and possible
pitfalls of AUC as well as appreciate the current relevance of past
work in the field. The book first introduces the basic principles
and technical setup of an AUC experiment and briefly describes the
optical systems used for detection. It then explores the
ultracentrifugation experiment from a macromolecular standpoint,
offering a detailed physical picture of the sedimentation process
and relevant macromolecular parameters. The authors present
important practical aspects for conducting an experiment, including
sample preparation, data acquisition and data structure, and the
execution of the centrifugal experiment. They also cover instrument
calibration and quality control experiments.
Analytical ultracentrifugation is one of the most powerful solution
techniques for the study of macromolecular interactions, to define
the number and stoichiometry of complexes formed, and to measure
affinities ranging from very strong to very weak and repulsive.
Building on the data analysis tools described in the volume
Sedimentation Velocity Analytical Ultracentrifugation: Discrete
Species and Size-Distributions of Macromolecules and Particles, and
the experimental and instrumental aspects in the first volume Basic
Principles of Analytical Ultracentrifugation, the present volume
Sedimentation Velocity Analytical Ultracentrifugation: Interacting
Systems is devoted to the theory and practical data analysis of
dynamically coupled sedimentation processes. This volume is
designed to fill a gap in biophysical methodology to provide a
framework that builds on the fundamentals of the highly developed
traditional methods of analytical ultracentrifugation, updated with
current methodology and from a viewpoint of modern applications. It
will be an invaluable resource for researchers and graduate
students interested in the application of analytical
ultracentrifugation in the study of interacting systems, such as
biological macromolecules, multi-protein complexes, polymers, or
nanoparticles.
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