Legionnaires disease, a potentially fatal type of pneumonia primarily affecting elderly and immuno-compromised persons, is caused by the ubiquitous environmental bacterium "Legionella pneumophila." This book offers authoritative reviews of different facets of its virulence, focusing on comparative phagocyte infection, virulence gene regulation, biochemical functions of effector proteins and cellular pathogen-host interactions, as well as host responses and immunity to "L. pneumophila." Taken together, the contributions in this compilation provide a state-of-the-art overview of current insights into the molecular pathogenesis of the opportunistic and potentially fatal pathogen "L. pneumophila.""

The methods described in the second edition on Legionella are for the study of distinct features of L. pneumophila. Chapters guide readers through ecology and physiology of legionella, legionella genetics, cellular microbiology of legionella, biochemical assays to study legionella effectors and enzymes, immunity and host response against legionella, metagenomics, proteomics, and host microbiomes of legionella. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Legionella: Methods and Protocols, Second Edition aims to be useful for scientists studying Legionella, and for a broader research community interested in the ecology, physiology, pathogenesis, immunity, genetics and evolution of other bacterial pathogens.

Legionella is a deadly pneumonia that has a high mortality rate among the elderly. The detailed protocols outlined in this new book in the 'Methods in Molecular Biology' series will extend our understanding of the bacterium and aid its elimination from our water supplies.

Legionnaires' disease, a potentially fatal type of pneumonia primarily affecting elderly and immuno-compromised persons, is caused by the ubiquitous environmental bacterium Legionella pneumophila. This book offers authoritative reviews of different facets of its virulence, focusing on comparative phagocyte infection, virulence gene regulation, biochemical functions of effector proteins and cellular pathogen-host interactions, as well as host responses and immunity to L. pneumophila. Taken together, the contributions in this compilation provide a state-of-the-art overview of current insights into the molecular pathogenesis of the opportunistic and potentially fatal pathogen L. pneumophila.

This Methods in Molecular Biology (TM) series book presents methods specifically adapted and developed for the study of distinct features of L. pneumophila. Includes materials lists, reproducible protocols, and notes on troubleshooting and pitfalls.