The methods described in the second edition on Legionella are for
the study of distinct features of L. pneumophila. Chapters guide
readers through ecology and physiology of legionella, legionella
genetics, cellular microbiology of legionella, biochemical assays
to study legionella effectors and enzymes, immunity and host
response against legionella, metagenomics, proteomics, and host
microbiomes of legionella. Written in the highly successful Methods
in Molecular Biology series format, chapters include introductions
to their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and tips on troubleshooting and avoiding known
pitfalls.Authoritative and cutting-edge, Legionella: Methods and
Protocols, Second Edition aims to be useful for scientists studying
Legionella, and for a broader research community interested in the
ecology, physiology, pathogenesis, immunity, genetics and evolution
of other bacterial pathogens.
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