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Matrix Metalloproteinase Protocols (Hardcover, 2nd ed. 2010): Ian M. Clark Matrix Metalloproteinase Protocols (Hardcover, 2nd ed. 2010)
Ian M. Clark; Adapted by David A Young, Andrew D. Rowan
R5,734 Discovery Miles 57 340 Ships in 10 - 15 working days

Since the discovery of a collagen-degrading protease in the tadpole tail in 1962, matrix metalloproteinase research has led to the discovery of more than twenty distinct vertebrate MMPs, along with a variety of homologues from diverse organisms such as the sea urchin, plants, insects, and nematode worms. Fully updating and adding to the popular first edition, Matrix Metalloproteinase Protocols, Second Edition includes a series of state-of-the-art techniques provided by eminent experts in the field. Beginning with a brief overview of the MMP arena, from how these enzymes fit into the larger degradome to what occurs when their expression and function in the mouse is modulated, the volume continues with sections on the expression and purification of MMPs and TIMPs, the detection of MMPs and TIMPs at both the protein and mRNA level, and our ability to assay MMP and TIMP activities in a wide variety of circumstances. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Matrix Metalloproteinase Protocols, Second Edition is an ideal source for many of the essential laboratory techniques for both novice and seasoned researchers alike collected in one convenient volume.

Matrix Metalloproteinase Protocols (Paperback, Softcover reprint of the original 2nd ed. 2010): Ian M. Clark Matrix Metalloproteinase Protocols (Paperback, Softcover reprint of the original 2nd ed. 2010)
Ian M. Clark; Adapted by David A Young, Andrew D. Rowan
R4,512 Discovery Miles 45 120 Ships in 10 - 15 working days

Since the discovery of a collagen-degrading protease in the tadpole tail in 1962, matrix metalloproteinase research has led to the discovery of more than twenty distinct vertebrate MMPs, along with a variety of homologues from diverse organisms such as the sea urchin, plants, insects, and nematode worms. Fully updating and adding to the popular first edition, Matrix Metalloproteinase Protocols, Second Edition includes a series of state-of-the-art techniques provided by eminent experts in the field. Beginning with a brief overview of the MMP arena, from how these enzymes fit into the larger degradome to what occurs when their expression and function in the mouse is modulated, the volume continues with sections on the expression and purification of MMPs and TIMPs, the detection of MMPs and TIMPs at both the protein and mRNA level, and our ability to assay MMP and TIMP activities in a wide variety of circumstances. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Matrix Metalloproteinase Protocols, Second Edition is an ideal source for many of the essential laboratory techniques for both novice and seasoned researchers alike collected in one convenient volume.

Plains of the Past - Book II of the Elder Earth Saga (Paperback): Ian M. Clark Plains of the Past - Book II of the Elder Earth Saga (Paperback)
Ian M. Clark
R387 Discovery Miles 3 870 Ships in 10 - 15 working days

The exciting conclusion to the Elder Earth Saga that began in "Prophecy of Shadows " The outcast warrior known as K'het tracks the immortal necromancer responsible for the murder of his mother and best friend. The old wizard has journeyed to the Plains of the Past, seeking to put an end to all living things on Elder Earth itself by reuniting the Geminus, one being of pure light, the other of pure shadow. The union of these exiled creatures will cause a cataclysm that would scorch the very heavens and leave all of Elder Earth a barren wasteland. K'het's path will take him through tests of the mind and body in a land where magical energies are still at play and legendary beasts still roam. K'het seeks the aid of allies in the elf kingdom of Tanglewood Forest. He will need their wisdom to catch the necromancer in time and face his destiny.

Prophecy of Shadows - Book I of the Elder Earth Saga (Paperback): Ian M. Clark Prophecy of Shadows - Book I of the Elder Earth Saga (Paperback)
Ian M. Clark
R416 Discovery Miles 4 160 Ships in 10 - 15 working days
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