Matrix Metalloproteinase Protocols (Paperback, Softcover reprint of the original 2nd ed. 2010)

Since the discovery of a collagen-degrading protease in the tadpole tail in 1962, matrix metalloproteinase research has led to the discovery of more than twenty distinct vertebrate MMPs, along with a variety of homologues from diverse organisms such as the sea urchin, plants, insects, and nematode worms. Fully updating and adding to the popular first edition, Matrix Metalloproteinase Protocols, Second Edition includes a series of state-of-the-art techniques provided by eminent experts in the field. Beginning with a brief overview of the MMP arena, from how these enzymes fit into the larger degradome to what occurs when their expression and function in the mouse is modulated, the volume continues with sections on the expression and purification of MMPs and TIMPs, the detection of MMPs and TIMPs at both the protein and mRNA level, and our ability to assay MMP and TIMP activities in a wide variety of circumstances. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Matrix Metalloproteinase Protocols, Second Edition is an ideal source for many of the essential laboratory techniques for both novice and seasoned researchers alike collected in one convenient volume.

General

Imprint:	HumanaPress
Country of origin:	United States
Series:	Methods in Molecular Biology, 622
Release date:	August 2016
First published:	2010
Editors:	Ian M. Clark
Adapted by:	David A Young • Andrew D. Rowan
Dimensions:	254 x 178mm (L x W)
Format:	Paperback
Pages:	492
Edition:	Softcover reprint of the original 2nd ed. 2010
ISBN-13:	978-1-4939-5622-7
Categories:	Books > Reference & Interdisciplinary > Interdisciplinary studies > General Books > Science & Mathematics > Biology, life sciences > Biochemistry > Enzymology Promotions
LSN:	1-4939-5622-1
Barcode:	9781493956227

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