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Books > Science & Mathematics > Biology, life sciences > Biochemistry > Enzymology
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Matrix Metalloproteinase Protocols (Paperback, Softcover reprint of the original 2nd ed. 2010)
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Matrix Metalloproteinase Protocols (Paperback, Softcover reprint of the original 2nd ed. 2010)
Series: Methods in Molecular Biology, 622
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Since the discovery of a collagen-degrading protease in the tadpole
tail in 1962, matrix metalloproteinase research has led to the
discovery of more than twenty distinct vertebrate MMPs, along with
a variety of homologues from diverse organisms such as the sea
urchin, plants, insects, and nematode worms. Fully updating and
adding to the popular first edition, Matrix Metalloproteinase
Protocols, Second Edition includes a series of state-of-the-art
techniques provided by eminent experts in the field. Beginning with
a brief overview of the MMP arena, from how these enzymes fit into
the larger degradome to what occurs when their expression and
function in the mouse is modulated, the volume continues with
sections on the expression and purification of MMPs and TIMPs, the
detection of MMPs and TIMPs at both the protein and mRNA level, and
our ability to assay MMP and TIMP activities in a wide variety of
circumstances. Written in the highly successful Methods in
Molecular Biology (TM) series format, chapters contain
introductions to their respective topics, lists of the necessary
materials and reagents, step-by-step, readily reproducible
laboratory protocols, and notes on troubleshooting and avoiding
known pitfalls. Comprehensive and cutting-edge, Matrix
Metalloproteinase Protocols, Second Edition is an ideal source for
many of the essential laboratory techniques for both novice and
seasoned researchers alike collected in one convenient volume.
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