Millions of people are suffering from severe condition of celiac disease. Different cereal grains like barley, rye and wheat contain proteins which are difficult to digest. Sorghum can be utilized as an alternative to wheat flour because it is gluten free. The protein present in sorghum, which is approximately 11.3% of the grain, is harmless for the celiac patients as well for the individuals with changing proportion of gluten intolerance. The major protein in sorghum is kafirin and it has many structural and functional attributes. It also provides the nutritional properties to the food products. The present study was an effort to explore chemical composition of three sorghum varieties named F-114, JS-2002 and Chengari according to respective methods. Sorghum protein kafirin was isolated from the varieties of sorghum and then characterized thorough SDS-PAGE. The isolated kafirin was added in composite flour of wheat and sorghum at the levels of 2 and 4% to make the gluten free bread.

Germinated grain was used in China not only for food but for medicine 5000 years ago. Germination is a natural biological process of plants by which the seeds come out of latency stage. Germination has profound effect on nutritional quality of the cereal. An advance treatment choice for CD is 'Protease therapy' which uses particular protein hydrolysis enzymes to breakdown gluten peptides which are rich in proline content into small peptides which are not harmful to intestinal T. Germinated cereal proteases have distinctive benefits when compared to microbial and fungal peptidases. They are very good replacements to recombinant proteases especially to those patients of CD which are susceptible to those proteases and whose cost of production is very high