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The technique of needle microinjection has become a prominent
experimental approach in biological research. Cellular organelles,
DNA and RNA, enzymes, structural proteins, metabolites, ions and
antibodies are just some of the molecular and cellular elements
that have been transposed from test tubes into living cells by
needle injection. This technique is broadly used as a valuable tool
for the study of many different cell responses in a variety of
systems. This text presents information on the technique of needle
microinjection's principles, the required equipment, preparation of
receiving cells and injected material and the analysis of the
results. It provides detailed protocols for a wide range of
important applications and different cellular systems from the
fields of cell cycle regulation, signal transduction, study of
transcriptional regulations, cytoskeletal functions, secretion and
intracellular transport.
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